Repetition-rate-dependent oxygen consumption modifies cytotoxicity in photodynamic therapy using pulsed light

Satoko Kawauchi; Yuji Morimoto; Hiroshi Asanuma; Hiroyuki Sato; Tsunenori Arai; Shunichi Sato; Isao Sakata; Takeshi Takemura; Susumu Nakajima; Makoto Kikuchi

doi:10.1117/12.474150

Repetition-rate-dependent oxygen consumption modifies cytotoxicity in photodynamic therapy using pulsed light

Satoko Kawauchi, Yuji Morimoto, Hiroshi Asanuma, Hiroyuki Sato, Tsunenori Arai, Shunichi Sato, Isao Sakata, Takeshi Takemura, Susumu Nakajima, Makoto Kikuchi

Department of Applied Physics and Physico-Informatics

Research output: Contribution to journal › Conference article › peer-review

Abstract

We studied the repetition-rate dependence of PDT cytotoxicity and relation between PDT cytotoxicity and both oxygen consumption and photobleaching during PDT in vitro. Mice renal carcinoma cells were incubated with second-generation photosensitizer, PAD-S31, and were irradiated with 670-nm nanoseconds pulsed light from YAG-OPO system. Four repetition rates of 30, 15, 5 and 3 Hz were investigated, provided that the incident peak intensity and the total light dose adjusted to 1.2 MW/cm² and 40 J/cm², respectively. We found limited cytotoxicity about 40% at 30 and 15 Hz and sufficient cytotoxicity about 80% at 5 and 3 Hz. The oxygen measurement during irradiation revealed that the 5- and 3-Hz irradiation caused slow oxygen consumption, while rapid oxygen consumption followed by a rapid recovery of oxygenation at 30 and 15 Hz. Interestingly, the fluorescence measurement during irradiation also demonstrated that photobleaching discontinued in the same period of oxygen recovery at 30 and 15 Hz. These discontinued oxygen consumptiona and photobleaching at 30 and 15 Hz might have limited effective total fluence and resulted in suppressed cytotoxicity. These results suggest that the PDT efficacy using a pulsed laser significantly depends on the pulse repetition rate probably due to different oxygen consumption process during PDT.

Original language	English
Pages (from-to)	125-130
Number of pages	6
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	4952
DOIs	https://doi.org/10.1117/12.474150
Publication status	Published - 2003 Sept 29
Event	Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XII - San Jose, CA, United States Duration: 2003 Jan 25 → 2003 Jan 26

Keywords

Oxygen consumption
PAD-S31
Photobleaching
Photodynamic therapy
Repetition rate

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

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10.1117/12.474150

Cite this

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title = "Repetition-rate-dependent oxygen consumption modifies cytotoxicity in photodynamic therapy using pulsed light",

abstract = "We studied the repetition-rate dependence of PDT cytotoxicity and relation between PDT cytotoxicity and both oxygen consumption and photobleaching during PDT in vitro. Mice renal carcinoma cells were incubated with second-generation photosensitizer, PAD-S31, and were irradiated with 670-nm nanoseconds pulsed light from YAG-OPO system. Four repetition rates of 30, 15, 5 and 3 Hz were investigated, provided that the incident peak intensity and the total light dose adjusted to 1.2 MW/cm2 and 40 J/cm2, respectively. We found limited cytotoxicity about 40% at 30 and 15 Hz and sufficient cytotoxicity about 80% at 5 and 3 Hz. The oxygen measurement during irradiation revealed that the 5- and 3-Hz irradiation caused slow oxygen consumption, while rapid oxygen consumption followed by a rapid recovery of oxygenation at 30 and 15 Hz. Interestingly, the fluorescence measurement during irradiation also demonstrated that photobleaching discontinued in the same period of oxygen recovery at 30 and 15 Hz. These discontinued oxygen consumptiona and photobleaching at 30 and 15 Hz might have limited effective total fluence and resulted in suppressed cytotoxicity. These results suggest that the PDT efficacy using a pulsed laser significantly depends on the pulse repetition rate probably due to different oxygen consumption process during PDT.",

keywords = "Oxygen consumption, PAD-S31, Photobleaching, Photodynamic therapy, Repetition rate",

author = "Satoko Kawauchi and Yuji Morimoto and Hiroshi Asanuma and Hiroyuki Sato and Tsunenori Arai and Shunichi Sato and Isao Sakata and Takeshi Takemura and Susumu Nakajima and Makoto Kikuchi",

year = "2003",

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doi = "10.1117/12.474150",

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note = "Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XII ; Conference date: 25-01-2003 Through 26-01-2003",

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TY - JOUR

T1 - Repetition-rate-dependent oxygen consumption modifies cytotoxicity in photodynamic therapy using pulsed light

AU - Kawauchi, Satoko

AU - Morimoto, Yuji

AU - Asanuma, Hiroshi

AU - Sato, Hiroyuki

AU - Arai, Tsunenori

AU - Sato, Shunichi

AU - Sakata, Isao

AU - Takemura, Takeshi

AU - Nakajima, Susumu

AU - Kikuchi, Makoto

PY - 2003/9/29

Y1 - 2003/9/29

N2 - We studied the repetition-rate dependence of PDT cytotoxicity and relation between PDT cytotoxicity and both oxygen consumption and photobleaching during PDT in vitro. Mice renal carcinoma cells were incubated with second-generation photosensitizer, PAD-S31, and were irradiated with 670-nm nanoseconds pulsed light from YAG-OPO system. Four repetition rates of 30, 15, 5 and 3 Hz were investigated, provided that the incident peak intensity and the total light dose adjusted to 1.2 MW/cm2 and 40 J/cm2, respectively. We found limited cytotoxicity about 40% at 30 and 15 Hz and sufficient cytotoxicity about 80% at 5 and 3 Hz. The oxygen measurement during irradiation revealed that the 5- and 3-Hz irradiation caused slow oxygen consumption, while rapid oxygen consumption followed by a rapid recovery of oxygenation at 30 and 15 Hz. Interestingly, the fluorescence measurement during irradiation also demonstrated that photobleaching discontinued in the same period of oxygen recovery at 30 and 15 Hz. These discontinued oxygen consumptiona and photobleaching at 30 and 15 Hz might have limited effective total fluence and resulted in suppressed cytotoxicity. These results suggest that the PDT efficacy using a pulsed laser significantly depends on the pulse repetition rate probably due to different oxygen consumption process during PDT.

AB - We studied the repetition-rate dependence of PDT cytotoxicity and relation between PDT cytotoxicity and both oxygen consumption and photobleaching during PDT in vitro. Mice renal carcinoma cells were incubated with second-generation photosensitizer, PAD-S31, and were irradiated with 670-nm nanoseconds pulsed light from YAG-OPO system. Four repetition rates of 30, 15, 5 and 3 Hz were investigated, provided that the incident peak intensity and the total light dose adjusted to 1.2 MW/cm2 and 40 J/cm2, respectively. We found limited cytotoxicity about 40% at 30 and 15 Hz and sufficient cytotoxicity about 80% at 5 and 3 Hz. The oxygen measurement during irradiation revealed that the 5- and 3-Hz irradiation caused slow oxygen consumption, while rapid oxygen consumption followed by a rapid recovery of oxygenation at 30 and 15 Hz. Interestingly, the fluorescence measurement during irradiation also demonstrated that photobleaching discontinued in the same period of oxygen recovery at 30 and 15 Hz. These discontinued oxygen consumptiona and photobleaching at 30 and 15 Hz might have limited effective total fluence and resulted in suppressed cytotoxicity. These results suggest that the PDT efficacy using a pulsed laser significantly depends on the pulse repetition rate probably due to different oxygen consumption process during PDT.

KW - Oxygen consumption

KW - PAD-S31

KW - Photobleaching

KW - Photodynamic therapy

KW - Repetition rate

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JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

T2 - Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XII

Y2 - 25 January 2003 through 26 January 2003

ER -

Repetition-rate-dependent oxygen consumption modifies cytotoxicity in photodynamic therapy using pulsed light

Abstract

Keywords

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