TY - JOUR
T1 - Replication study for the association of new meta-analysis-derived risk loci with susceptibility to type 2 diabetes in 6,244 Japanese individuals
AU - Omori, S.
AU - Tanaka, Y.
AU - Horikoshi, M.
AU - Takahashi, A.
AU - Hara, K.
AU - Hirose, H.
AU - Kashiwagi, A.
AU - Kaku, K.
AU - Kawamori, R.
AU - Kadowaki, T.
AU - Nakamura, Y.
AU - Maeda, S.
N1 - Funding Information:
Acknowledgements We thank K. Hotta, S. Tsukada and H. Unoki at the Laboratory for Endocrinology and Metabolism, RIKEN Center for Genomic Medicine for helpful discussions and thank the technical staff of the Laboratory for Endocrinology and Metabolism, RIKEN Center for Genomic Medicine, for their technical assistance. This work was partly supported by a Grant-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology, Japan (to S. Maeda).
PY - 2009/8
Y1 - 2009/8
N2 - Additional susceptibility loci for type 2 diabetes have been identified by a meta-analysis of genome-wide association studies (GWASs) in European populations. To examine further the roles of these new loci, we performed a replication study for the association of these single-nucleotide polymorphism (SNP) loci with the disease in three independent Japanese populations. We genotyped seven of the 11 SNPs that emerged in stage 2 of the meta-analysis for European GWASs (rs864745 in JAZF1, rs12779790 near CDC123/CAMK1D, rs7961581 near TSPAN8/LGR5, rs4607103 near ADAMTS9, rs10923931 in NOTCH2, rs1153188 near DCD and rs9472138 near VEGFA) for three independent Japanese populations (first set, 1,630 type 2 diabetes patients vs 1,064 controls; second set, 1,272 type 2 diabetes patients vs 856 controls; third set, 486 type 2 diabetes patients vs 936 controls) using a TaqMan assay. The association of the SNP loci in each population was analysed using a logistic regression analysis, adjusting for age, sex and BMI, and the data were evaluated by a meta-analysis. A meta-analysis for the three case-control studies identified a nominal association of rs864745 in JAZF1 with type 2 diabetes (OR 1.148, 95% CI 1.034-1.275, p∈=∈0.0098, corrected p∈=∈0.069). The association of other loci did not reach statistically significant levels (nominal p∈>∈0.05). From these results the contribution of these seven loci in conferring susceptibility to type 2 diabetes is considered minor in the Japanese population, if they are present.
AB - Additional susceptibility loci for type 2 diabetes have been identified by a meta-analysis of genome-wide association studies (GWASs) in European populations. To examine further the roles of these new loci, we performed a replication study for the association of these single-nucleotide polymorphism (SNP) loci with the disease in three independent Japanese populations. We genotyped seven of the 11 SNPs that emerged in stage 2 of the meta-analysis for European GWASs (rs864745 in JAZF1, rs12779790 near CDC123/CAMK1D, rs7961581 near TSPAN8/LGR5, rs4607103 near ADAMTS9, rs10923931 in NOTCH2, rs1153188 near DCD and rs9472138 near VEGFA) for three independent Japanese populations (first set, 1,630 type 2 diabetes patients vs 1,064 controls; second set, 1,272 type 2 diabetes patients vs 856 controls; third set, 486 type 2 diabetes patients vs 936 controls) using a TaqMan assay. The association of the SNP loci in each population was analysed using a logistic regression analysis, adjusting for age, sex and BMI, and the data were evaluated by a meta-analysis. A meta-analysis for the three case-control studies identified a nominal association of rs864745 in JAZF1 with type 2 diabetes (OR 1.148, 95% CI 1.034-1.275, p∈=∈0.0098, corrected p∈=∈0.069). The association of other loci did not reach statistically significant levels (nominal p∈>∈0.05). From these results the contribution of these seven loci in conferring susceptibility to type 2 diabetes is considered minor in the Japanese population, if they are present.
KW - Association study
KW - Japanese
KW - SNP
KW - Type 2 diabetes
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U2 - 10.1007/s00125-009-1397-5
DO - 10.1007/s00125-009-1397-5
M3 - Article
C2 - 19455301
AN - SCOPUS:67650655994
SN - 0012-186X
VL - 52
SP - 1554
EP - 1560
JO - Diabetologia
JF - Diabetologia
IS - 8
ER -
