Requirement of full TCR repertoire for regulatory T cells to maintain intestinal homeostasis

Junko Nishio, Minato Baba, Koji Atarashi, Takeshi Tanoue, Hideo Negishi, Hideyuki Yanai, Sonoko Habu, Shohei Hori, Kenya Honda, Tadatsugu Taniguchi

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The regulation of intestinal homeostasis by the immune system involves the dynamic interplay between gut commensal microbiota and resident immune cells. It is well known that a large and diverse lymphocyte antigen receptor repertoire enables the immune system to recognize and respond to a wide range of invading pathogens. There is also an emerging appreciation for a critical role the T-cell receptor (TCR) repertoire serves in the maintenance of peripheral tolerance by regulatory T cells (Tregs). Nevertheless, how the diversity of the TCR repertoire in Tregs affects intestinal homeostasis remains unknown. To address this question, we studied mice whose T cells express a restricted TCR repertoire. We observed the development of spontaneous colitis, accompanied by the induction of T-helper type 17 cells in the colon that is driven by gut commensal microbiota. We provide further evidence that a restricted TCR repertoire causes a loss of tolerogenicity to microbiota, accompanied by a paucity of peripherally derived, Helios- Tregs and hyperactivation of migratory dendritic cells. These results thus reveal a new facet of the TCR repertoire in which Tregs require a diverse TCR repitoire for intestinal homeostasis, suggesting an additional driving force in the evolutional significance of the TCR repertoire.

Original languageEnglish
Pages (from-to)12770-12775
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume112
Issue number41
DOIs
Publication statusPublished - 2015 Oct 13

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Regulatory T-Lymphocytes
T-Cell Antigen Receptor
Homeostasis
Immune System
Peripheral Tolerance
Th17 Cells
Antigen Receptors
Microbiota
Colitis
Dendritic Cells
Colon
Maintenance
Lymphocytes
T-Lymphocytes

Keywords

  • Colitis
  • Helios
  • Migratory DCs
  • TCR repertoire
  • Treg

ASJC Scopus subject areas

  • General

Cite this

Requirement of full TCR repertoire for regulatory T cells to maintain intestinal homeostasis. / Nishio, Junko; Baba, Minato; Atarashi, Koji; Tanoue, Takeshi; Negishi, Hideo; Yanai, Hideyuki; Habu, Sonoko; Hori, Shohei; Honda, Kenya; Taniguchi, Tadatsugu.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 112, No. 41, 13.10.2015, p. 12770-12775.

Research output: Contribution to journalArticle

Nishio, Junko ; Baba, Minato ; Atarashi, Koji ; Tanoue, Takeshi ; Negishi, Hideo ; Yanai, Hideyuki ; Habu, Sonoko ; Hori, Shohei ; Honda, Kenya ; Taniguchi, Tadatsugu. / Requirement of full TCR repertoire for regulatory T cells to maintain intestinal homeostasis. In: Proceedings of the National Academy of Sciences of the United States of America. 2015 ; Vol. 112, No. 41. pp. 12770-12775.
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