Reshuffling of the Bacillus subtilis 168 genome by multifold inversion

Azusa Kuroki, Tsutomu Toda, Kuniko Matsui, Rie Uotsu-Tomita, Masaru Tomita, Mitsuhiro Itaya

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The genome of Bacillus subtilis 168 was modified to yield a genome vector for the cloning of DNA several Mb in size. Unlike contemporary plasmid-based vectors, this 4.2 Mb genome vector requires specific in vivo handling protocols because of its large size. Inversion mutagenesis, a method to modify local genome structure without gain or loss of genes, was applied intensively to the B. subtilis genome; this technique made possible both exchange and translocation of designated regions of the genome. This method not only reshuffles the genome of B. subtilis, but can provide insight into the biologic principles underlying genome plasticity.

Original languageEnglish
Pages (from-to)97-105
Number of pages9
JournalJournal of biochemistry
Volume143
Issue number1
DOIs
Publication statusPublished - 2008 Jan 1

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Keywords

  • Antibiotic resistance
  • Competence
  • Homologous recombination
  • Inversion
  • Transformation
  • Translocation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Kuroki, A., Toda, T., Matsui, K., Uotsu-Tomita, R., Tomita, M., & Itaya, M. (2008). Reshuffling of the Bacillus subtilis 168 genome by multifold inversion. Journal of biochemistry, 143(1), 97-105. https://doi.org/10.1093/jb/mvm197