Resident endothelial cells surrounding damaged arterial endothelium reendothelialize the lesion

Yoshiaki Itoh, Haruki Toriumi, Satoshi Yamada, Haruhiko Hoshino, Norihiro Suzuki

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Objective: To evaluate endothelial repair processes in denuded pial vessels to clarify mechanisms for reconstructing endothelium (because endothelial repair of the cerebral artery after its damage is critical for the prevention of thrombosis, the maintenance of vascular tone, and the protection of the brain by the blood-brain barrier). Methods and Results: Endothelial cells (ECs) in a 350-μm-long segment of the middle cerebral artery were damaged through a photochemical reaction. Tie2-green fluorescent protein transgenic mice were used for the identification of ECs. Six hours after the endothelial damage, ECs were detached from the luminal surface of the damaged artery, which was then covered with a platelet carpet. Within 24 hours, recovery of the denuded artery started at both edges, with EC elongation and migration. The repair rate was faster at the proximal edge than at the distal edge. Reendothelialization with EC proliferation peaked at 2 to 3 days and ended at 5 days, together with normalization of EC length, with no apparent involvement of foreign progenitor cells. Conclusion: Our in vivo study demonstrated a stepwise reendothelialization process by resident ECs of the pial artery. The prevention of thrombosis, vasospasm, and treatment for blood-brain barrier dysfunction should be considered during the reendothelialization period.

Original languageEnglish
Pages (from-to)1725-1732
Number of pages8
JournalArteriosclerosis, Thrombosis, and Vascular Biology
Volume30
Issue number9
DOIs
Publication statusPublished - 2010 Sep

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Endothelium
Endothelial Cells
Blood-Brain Barrier
Arteries
Thrombosis
Cerebral Arteries
Middle Cerebral Artery
Green Fluorescent Proteins
Transgenic Mice
Cell Movement
Blood Vessels
Stem Cells
Blood Platelets
Maintenance
Cell Proliferation

Keywords

  • confocal laser microscopy
  • photochemical reaction
  • pial artery
  • reendothelialization
  • Tie2

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Medicine(all)

Cite this

Resident endothelial cells surrounding damaged arterial endothelium reendothelialize the lesion. / Itoh, Yoshiaki; Toriumi, Haruki; Yamada, Satoshi; Hoshino, Haruhiko; Suzuki, Norihiro.

In: Arteriosclerosis, Thrombosis, and Vascular Biology, Vol. 30, No. 9, 09.2010, p. 1725-1732.

Research output: Contribution to journalArticle

Itoh, Yoshiaki ; Toriumi, Haruki ; Yamada, Satoshi ; Hoshino, Haruhiko ; Suzuki, Norihiro. / Resident endothelial cells surrounding damaged arterial endothelium reendothelialize the lesion. In: Arteriosclerosis, Thrombosis, and Vascular Biology. 2010 ; Vol. 30, No. 9. pp. 1725-1732.
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