Resolvin E1 inhibits dendritic cell migration in the skin and attenuates contact hypersensitivity responses

Yu Sawada, Tetsuya Honda, Sho Hanakawa, Satoshi Nakamizo, Teruasa Murata, Yuri Ueharaguchi-Tanada, Sachiko Ono, Wataru Amano, Saeko Nakajima, Gyohei Egawa, Hideaki Tanizaki, Atsushi Otsuka, Akihiko Kitoh, Teruki Dainichi, Narihito Ogawa, Yuichi Kobayashi, Takehiko Yokomizo, Makoto Arita, Motonobu Nakamura, Yoshiki Miyachi & 1 others Kenji Kabashima

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Resolvin E1 (RvE1) is a lipid mediator derived from ?3 polyunsaturated fatty acids that exerts potent antiinflammatory roles in several murine models. The antiinflammatory mechanism of RvE1 in acquired immune responses has been attributed to attenuation of cytokine production by dendritic cells (DCs). In this study, we newly investigated the effect of RvE1 on DC motility using two-photon microscopy in a contact hypersensitivity (CHS) model and found that RvE1 impaired DC motility in the skin. In addition, RvE1 attenuated T cell priming in the draining lymph nodes and effector T cell activation in the skin, which led to the reduced skin inflammation in CHS. In contrast, leukotriene B4 (LTB4) induced actin filament reorganization in DCs and increased DC motility by activating Cdc42 and Rac1 via BLT1, which was abrogated by RvE1. Collectively, our results suggest that RvE1 attenuates cutaneous acquired immune responses by inhibiting cutaneous DC motility, possibly through LTB4-BLT1 signaling blockade.

Original languageEnglish
Pages (from-to)1921-1930
Number of pages10
JournalJournal of Experimental Medicine
Volume212
Issue number11
DOIs
Publication statusPublished - 2015
Externally publishedYes

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Contact Dermatitis
Dendritic Cells
Cell Movement
Skin
Leukotriene B4
Anti-Inflammatory Agents
T-Lymphocytes
Langerhans Cells
5S,12R,18R-trihydroxy-6Z,8E,10E,14Z,16E-eicosapentaenoic acid
Unsaturated Fatty Acids
Actin Cytoskeleton
Photons
Microscopy
Lymph Nodes
Cytokines
Inflammation
Lipids

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Sawada, Y., Honda, T., Hanakawa, S., Nakamizo, S., Murata, T., Ueharaguchi-Tanada, Y., ... Kabashima, K. (2015). Resolvin E1 inhibits dendritic cell migration in the skin and attenuates contact hypersensitivity responses. Journal of Experimental Medicine, 212(11), 1921-1930. https://doi.org/10.1084/jem.20150381

Resolvin E1 inhibits dendritic cell migration in the skin and attenuates contact hypersensitivity responses. / Sawada, Yu; Honda, Tetsuya; Hanakawa, Sho; Nakamizo, Satoshi; Murata, Teruasa; Ueharaguchi-Tanada, Yuri; Ono, Sachiko; Amano, Wataru; Nakajima, Saeko; Egawa, Gyohei; Tanizaki, Hideaki; Otsuka, Atsushi; Kitoh, Akihiko; Dainichi, Teruki; Ogawa, Narihito; Kobayashi, Yuichi; Yokomizo, Takehiko; Arita, Makoto; Nakamura, Motonobu; Miyachi, Yoshiki; Kabashima, Kenji.

In: Journal of Experimental Medicine, Vol. 212, No. 11, 2015, p. 1921-1930.

Research output: Contribution to journalArticle

Sawada, Y, Honda, T, Hanakawa, S, Nakamizo, S, Murata, T, Ueharaguchi-Tanada, Y, Ono, S, Amano, W, Nakajima, S, Egawa, G, Tanizaki, H, Otsuka, A, Kitoh, A, Dainichi, T, Ogawa, N, Kobayashi, Y, Yokomizo, T, Arita, M, Nakamura, M, Miyachi, Y & Kabashima, K 2015, 'Resolvin E1 inhibits dendritic cell migration in the skin and attenuates contact hypersensitivity responses', Journal of Experimental Medicine, vol. 212, no. 11, pp. 1921-1930. https://doi.org/10.1084/jem.20150381
Sawada, Yu ; Honda, Tetsuya ; Hanakawa, Sho ; Nakamizo, Satoshi ; Murata, Teruasa ; Ueharaguchi-Tanada, Yuri ; Ono, Sachiko ; Amano, Wataru ; Nakajima, Saeko ; Egawa, Gyohei ; Tanizaki, Hideaki ; Otsuka, Atsushi ; Kitoh, Akihiko ; Dainichi, Teruki ; Ogawa, Narihito ; Kobayashi, Yuichi ; Yokomizo, Takehiko ; Arita, Makoto ; Nakamura, Motonobu ; Miyachi, Yoshiki ; Kabashima, Kenji. / Resolvin E1 inhibits dendritic cell migration in the skin and attenuates contact hypersensitivity responses. In: Journal of Experimental Medicine. 2015 ; Vol. 212, No. 11. pp. 1921-1930.
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