Resolvin E1 maintains macrophage function under cigarette smoke-induced oxidative stress

Rina Takamiya, Koichi Fukunaga, Makoto Arita, Jun Miyata, Hiroyuki Seki, Naoto Minematsu, Makoto Suematsu, Koichiro Asano

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Cigarette smoke (CS) induces oxidative stress, which disables macrophage function. In this study, we examined whether Resolvin E1 (RvE1), a pro-resolving mediator known to enhance macrophage functions, attenuates the damage of macrophages by CS extract (CSE) induced oxidative stress. RvE1 blocked p47phox translocation to plasma membrane induced by CSE in a macrophage cell line, RAW264.7 cells, resulting in suppression of superoxide production. Furthermore, pretreatment of RAW264.7 cells with RvE1 restored the phagocytic activity and reduced cell death induced by treatment of CSE. These results suggest that RvE1 plays important roles in preserving macrophage function under CS-induced oxidative stress.

Original languageEnglish
Pages (from-to)328-333
Number of pages6
JournalFEBS Open Bio
Volume2
DOIs
Publication statusPublished - 2012

Fingerprint

Oxidative stress
Macrophages
Smoke
Tobacco Products
Oxidative Stress
Cell death
Cell membranes
Superoxides
Cell Death
Cells
Cell Membrane
5S,12R,18R-trihydroxy-6Z,8E,10E,14Z,16E-eicosapentaenoic acid
Cell Line

Keywords

  • Cigarette smoke
  • Macrophage
  • Reactive oxygen species
  • Resolvin E1

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Resolvin E1 maintains macrophage function under cigarette smoke-induced oxidative stress. / Takamiya, Rina; Fukunaga, Koichi; Arita, Makoto; Miyata, Jun; Seki, Hiroyuki; Minematsu, Naoto; Suematsu, Makoto; Asano, Koichiro.

In: FEBS Open Bio, Vol. 2, 2012, p. 328-333.

Research output: Contribution to journalArticle

Takamiya, Rina ; Fukunaga, Koichi ; Arita, Makoto ; Miyata, Jun ; Seki, Hiroyuki ; Minematsu, Naoto ; Suematsu, Makoto ; Asano, Koichiro. / Resolvin E1 maintains macrophage function under cigarette smoke-induced oxidative stress. In: FEBS Open Bio. 2012 ; Vol. 2. pp. 328-333.
@article{f3287b1e7be94294be7604b339978ef9,
title = "Resolvin E1 maintains macrophage function under cigarette smoke-induced oxidative stress",
abstract = "Cigarette smoke (CS) induces oxidative stress, which disables macrophage function. In this study, we examined whether Resolvin E1 (RvE1), a pro-resolving mediator known to enhance macrophage functions, attenuates the damage of macrophages by CS extract (CSE) induced oxidative stress. RvE1 blocked p47phox translocation to plasma membrane induced by CSE in a macrophage cell line, RAW264.7 cells, resulting in suppression of superoxide production. Furthermore, pretreatment of RAW264.7 cells with RvE1 restored the phagocytic activity and reduced cell death induced by treatment of CSE. These results suggest that RvE1 plays important roles in preserving macrophage function under CS-induced oxidative stress.",
keywords = "Cigarette smoke, Macrophage, Reactive oxygen species, Resolvin E1",
author = "Rina Takamiya and Koichi Fukunaga and Makoto Arita and Jun Miyata and Hiroyuki Seki and Naoto Minematsu and Makoto Suematsu and Koichiro Asano",
year = "2012",
doi = "10.1016/j.fob.2012.10.001",
language = "English",
volume = "2",
pages = "328--333",
journal = "FEBS Open Bio",
issn = "2211-5463",
publisher = "Elsevier BV",

}

TY - JOUR

T1 - Resolvin E1 maintains macrophage function under cigarette smoke-induced oxidative stress

AU - Takamiya, Rina

AU - Fukunaga, Koichi

AU - Arita, Makoto

AU - Miyata, Jun

AU - Seki, Hiroyuki

AU - Minematsu, Naoto

AU - Suematsu, Makoto

AU - Asano, Koichiro

PY - 2012

Y1 - 2012

N2 - Cigarette smoke (CS) induces oxidative stress, which disables macrophage function. In this study, we examined whether Resolvin E1 (RvE1), a pro-resolving mediator known to enhance macrophage functions, attenuates the damage of macrophages by CS extract (CSE) induced oxidative stress. RvE1 blocked p47phox translocation to plasma membrane induced by CSE in a macrophage cell line, RAW264.7 cells, resulting in suppression of superoxide production. Furthermore, pretreatment of RAW264.7 cells with RvE1 restored the phagocytic activity and reduced cell death induced by treatment of CSE. These results suggest that RvE1 plays important roles in preserving macrophage function under CS-induced oxidative stress.

AB - Cigarette smoke (CS) induces oxidative stress, which disables macrophage function. In this study, we examined whether Resolvin E1 (RvE1), a pro-resolving mediator known to enhance macrophage functions, attenuates the damage of macrophages by CS extract (CSE) induced oxidative stress. RvE1 blocked p47phox translocation to plasma membrane induced by CSE in a macrophage cell line, RAW264.7 cells, resulting in suppression of superoxide production. Furthermore, pretreatment of RAW264.7 cells with RvE1 restored the phagocytic activity and reduced cell death induced by treatment of CSE. These results suggest that RvE1 plays important roles in preserving macrophage function under CS-induced oxidative stress.

KW - Cigarette smoke

KW - Macrophage

KW - Reactive oxygen species

KW - Resolvin E1

UR - http://www.scopus.com/inward/record.url?scp=84867685794&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84867685794&partnerID=8YFLogxK

U2 - 10.1016/j.fob.2012.10.001

DO - 10.1016/j.fob.2012.10.001

M3 - Article

C2 - 23772366

AN - SCOPUS:84867685794

VL - 2

SP - 328

EP - 333

JO - FEBS Open Bio

JF - FEBS Open Bio

SN - 2211-5463

ER -