Resolvin E1 promotes mucosal surface clearance of neutrophils: A new paradigm for inflammatory resolution

Eric L. Campbell, Nancy A. Louis, Sarah E. Tomassetti, Geraldine O. Canny, Makoto Arita, Charles N. Serhan, Sean P. Colgan

Research output: Contribution to journalArticle

135 Citations (Scopus)

Abstract

Migration of neutrophils (PMN) across epithelia is a pathological hallmark of numerous mucosal diseases. Whereas lesions at mucosal surfaces are generally self-limiting, endogenous mechanisms of resolution are incompletely understood. Previous studies revealed that resolvins directly act on PMN to attenuate transendothelial migration, less is known about the influence of resolvins on PMN-epithelial interactions and whether they act on epithelia. We studied the dynamics of resolvin E1 (RvE1) actions on leukocyte transepithelial migration. PMN exposure to RvE1 or chemerin (peptide agonist of ChemR23) reduced transepithelial migration in a concentration-dependent manner. Conversely, activation of epithelial ChemR23 promoted apical clearance of PMN. A nonbiased screen of known PMN ligands expressed on epithelial cells in response to RvE1 revealed selective induction of CD55, an apically expressed antiadhesive molecule. CD55 promoter analysis demonstrated that both RvE1 and chemerin activate the CD55 promoter. Inhibition of CD55 by neutralizing antibody prevented RvE1-dependent augmentation of apical PMN clearance. Taken together these findings implicate a "two-hit" model of inflammatory resolution, whereby activation of the PMN RvE1 receptor attenuates transepithelial migration and subsequent actions on the epithelium promote CD55-dependent clearance of PMN across the epithelial cell surface promoting active inflammatory resolution.

Original languageEnglish
Pages (from-to)3162-3170
Number of pages9
JournalFASEB Journal
Volume21
Issue number12
DOIs
Publication statusPublished - 2007 Oct
Externally publishedYes

Fingerprint

Transendothelial and Transepithelial Migration
neutrophils
Neutrophils
epithelium
epithelial cells
Epithelium
neutralizing antibodies
lesions (animal)
agonists
leukocytes
Epithelial Cells
Chemical activation
peptides
receptors
Neutralizing Antibodies
5S,12R,18R-trihydroxy-6Z,8E,10E,14Z,16E-eicosapentaenoic acid
Leukocytes
Ligands
Peptides
Molecules

Keywords

  • CD55
  • Epithelia
  • Icam-1 transmigration
  • Lipid mediator
  • Transmigration

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology
  • Medicine(all)

Cite this

Campbell, E. L., Louis, N. A., Tomassetti, S. E., Canny, G. O., Arita, M., Serhan, C. N., & Colgan, S. P. (2007). Resolvin E1 promotes mucosal surface clearance of neutrophils: A new paradigm for inflammatory resolution. FASEB Journal, 21(12), 3162-3170. https://doi.org/10.1096/fj.07-8473com

Resolvin E1 promotes mucosal surface clearance of neutrophils : A new paradigm for inflammatory resolution. / Campbell, Eric L.; Louis, Nancy A.; Tomassetti, Sarah E.; Canny, Geraldine O.; Arita, Makoto; Serhan, Charles N.; Colgan, Sean P.

In: FASEB Journal, Vol. 21, No. 12, 10.2007, p. 3162-3170.

Research output: Contribution to journalArticle

Campbell, EL, Louis, NA, Tomassetti, SE, Canny, GO, Arita, M, Serhan, CN & Colgan, SP 2007, 'Resolvin E1 promotes mucosal surface clearance of neutrophils: A new paradigm for inflammatory resolution', FASEB Journal, vol. 21, no. 12, pp. 3162-3170. https://doi.org/10.1096/fj.07-8473com
Campbell, Eric L. ; Louis, Nancy A. ; Tomassetti, Sarah E. ; Canny, Geraldine O. ; Arita, Makoto ; Serhan, Charles N. ; Colgan, Sean P. / Resolvin E1 promotes mucosal surface clearance of neutrophils : A new paradigm for inflammatory resolution. In: FASEB Journal. 2007 ; Vol. 21, No. 12. pp. 3162-3170.
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