TY - JOUR
T1 - Resolvin E2
T2 - Identification and Anti-Inflammatory Actions: Pivotal Role of Human 5-Lipoxygenase in Resolvin E Series Biosynthesis
AU - Tjonahen, Eric
AU - Oh, Sungwhan F.
AU - Siegelman, Jeffrey
AU - Elangovan, Siva
AU - Percarpio, Katherine B.
AU - Hong, Song
AU - Arita, Makoto
AU - Serhan, Charles N.
N1 - Funding Information:
We thank M.H. Small and Stacey Lindberg for assistance with manuscript preparation and Timothy F. Porter and Tamara Baer for technical assistance with murine protocols. The present studies were supported by National Institutes of Health grants P50-DE016191 and GM38765. C.N.S. is a consultant for the clinical development of lipoxin stable analogs with Berlex-Schering AG and Resolvyx Pharmaceuticals for development of resolvin E1.
PY - 2006/11
Y1 - 2006/11
N2 - The family of resolvins consists of omega-3 fatty acid-derived mediators, including E series resolvins generated from eicosapentaenoic acid (EPA), and carry potent anti-inflammatory properties. Here, we report the isolation, identification, and bioactions of resolvin E2 (RvE2), which is 5S,18-dihydroxy-eicosapentaenoic acid. RvE2 stopped zymosan-induced polymorphonuclear (PMN) leukocyte infiltration and displayed potent anti-inflammatory properties in murine peritonitis. We also demonstrate that human recombinant 5-lipoxygenase generates RvE2 from a common precursor of E series resolvins, namely, 18-hydroxyeicosapentaenoate (18-HEPE). Furthermore, the initial 5-hydroperoxide intermediate was also converted to a 5(6)-epoxide intermediate in RvE1 formation. These results demonstrate that RvE2, together with RvE1, may contribute to the beneficial actions of omega-3 fatty acids in human diseases. Moreover, they indicate that the 5-lipoxygenase in human leukocytes is a pivotal enzyme that can produce both pro- and anti-inflammatory chemical mediators.
AB - The family of resolvins consists of omega-3 fatty acid-derived mediators, including E series resolvins generated from eicosapentaenoic acid (EPA), and carry potent anti-inflammatory properties. Here, we report the isolation, identification, and bioactions of resolvin E2 (RvE2), which is 5S,18-dihydroxy-eicosapentaenoic acid. RvE2 stopped zymosan-induced polymorphonuclear (PMN) leukocyte infiltration and displayed potent anti-inflammatory properties in murine peritonitis. We also demonstrate that human recombinant 5-lipoxygenase generates RvE2 from a common precursor of E series resolvins, namely, 18-hydroxyeicosapentaenoate (18-HEPE). Furthermore, the initial 5-hydroperoxide intermediate was also converted to a 5(6)-epoxide intermediate in RvE1 formation. These results demonstrate that RvE2, together with RvE1, may contribute to the beneficial actions of omega-3 fatty acids in human diseases. Moreover, they indicate that the 5-lipoxygenase in human leukocytes is a pivotal enzyme that can produce both pro- and anti-inflammatory chemical mediators.
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U2 - 10.1016/j.chembiol.2006.09.011
DO - 10.1016/j.chembiol.2006.09.011
M3 - Article
C2 - 17114001
AN - SCOPUS:33751020561
VL - 13
SP - 1193
EP - 1202
JO - Cell Chemical Biology
JF - Cell Chemical Biology
SN - 2451-9448
IS - 11
ER -
