Resonance assignments, secondary structure and 15N relaxation data of the human transcriptional coactivator hMBF1 (57-148)

Masaki Mishima, Jun Ozaki, Takahisa Ikegami, Yasuaki Kabe, Masahide Goto, Hitoshi Ueda, Susumu Hirose, Hiroshi Handa, Masahiro Shirakawa

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Multiprotein bridging factor 1 (MBF1) is a transcriptional coactivator that is thought to bridge between the TATA box-binding protein (TBP) and DNA binding regulatory factors, and is conserved from yeast to human. Human MBF1 (hMBF1) can bind to TBP and to the nuclear receptor Ad4BP, and is suggested to mediate Ad4BP-dependent transcriptional activation. Here we report the resonance assignments and secondary structure of hMBF1 (57-148) that contains both TBP binding and activator binding residues. 15N relaxation data were also obtained. As a result, hMBF1 (57-148) was shown to consist of flexible N-terminal residues and a C-terminal domain. The C-terminal domain contains four helices and a conserved C-terminal region.

Original languageEnglish
Pages (from-to)373-376
Number of pages4
JournalJournal of Biomolecular NMR
Volume14
Issue number4
DOIs
Publication statusPublished - 1999
Externally publishedYes

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TATA-Box Binding Protein
Protein Binding
Cytoplasmic and Nuclear Receptors
Yeast
Chemical activation
Transcriptional Activation
Yeasts
DNA

Keywords

  • N relaxation
  • Resonance assignment
  • Secondary structure
  • Transcriptional coactivator

ASJC Scopus subject areas

  • Spectroscopy
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Resonance assignments, secondary structure and 15N relaxation data of the human transcriptional coactivator hMBF1 (57-148). / Mishima, Masaki; Ozaki, Jun; Ikegami, Takahisa; Kabe, Yasuaki; Goto, Masahide; Ueda, Hitoshi; Hirose, Susumu; Handa, Hiroshi; Shirakawa, Masahiro.

In: Journal of Biomolecular NMR, Vol. 14, No. 4, 1999, p. 373-376.

Research output: Contribution to journalArticle

Mishima, Masaki ; Ozaki, Jun ; Ikegami, Takahisa ; Kabe, Yasuaki ; Goto, Masahide ; Ueda, Hitoshi ; Hirose, Susumu ; Handa, Hiroshi ; Shirakawa, Masahiro. / Resonance assignments, secondary structure and 15N relaxation data of the human transcriptional coactivator hMBF1 (57-148). In: Journal of Biomolecular NMR. 1999 ; Vol. 14, No. 4. pp. 373-376.
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AU - Kabe, Yasuaki

AU - Goto, Masahide

AU - Ueda, Hitoshi

AU - Hirose, Susumu

AU - Handa, Hiroshi

AU - Shirakawa, Masahiro

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