Respiratory syncytial virus-induced interleukin-4 production by human conjunctival epithelial cells contributes to allergy

Preliminary study

Hiroshi Fujishima, Ichiro Saito, Yoshitaka Okamoto, Tsutomu Takeuchi, Kazuo Tsubota

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Purpose. We previously reported allergic conjunctivitis was associated with a local pattern of cytokines and identified respiratory syncytial virus (RSV) in the conjunctival epithelium, in patients with allergic conjunctivitis. We hypothesized that RSV infected the normal conjunctival epithelium and produced interleukin-4 (IL-4), causing an allergic reaction. To determine this event, we studied production of IL-4 by human conjunctival epithelial cells, after infection with RSV. Methods. Conjunctival samples were collected with a special brush and were challenged with 5 x 106 pfu/ml of live RSV. RSV infection was detected by immunocytochemical study and RT-PCR. HLA-DR antigen was expressed by flow cytometry. IL-4 was assayed by a sandwich ELISA. Results. Immunocytochemical study and RT-PCR showed that RSV infected the conjunctival epithelial cells. HLA-DR was demonstrated in the RSV-infected conjunctival epithelial cells. Average concentrations of IL-4 in culture supernatants in infected cells were 3.8 ± 1.8, 3.6 ± 1.1, and 5.4 ± 1.8 pg/ml at 24, 48, and 72 h, respectively. Levels in non-infected cells were 0.33 ± 0.0, 0.59 ± 0.69, and 0.4 ± 0.22 pg/ml, respectively (p > 0.001). Conclusion. Findings suggest that the expression of IL-4 is associated with intracellular infection by RSV in conjunctiva. With our former reports, RSV infection in conjunctival epithelial cells can be suggested as one of the possible environmental pathogens of allergic conjunctivitis.

Original languageEnglish
Pages (from-to)656-662
Number of pages7
JournalCurrent Eye Research
Volume17
Issue number6
DOIs
Publication statusPublished - 1998

Fingerprint

Respiratory Syncytial Viruses
Respiratory Syncytial Virus Infections
Interleukin-4
Hypersensitivity
Allergic Conjunctivitis
Epithelial Cells
HLA-DR Antigens
Epithelium
Polymerase Chain Reaction
Conjunctiva
Flow Cytometry
Enzyme-Linked Immunosorbent Assay
Cytokines

Keywords

  • Allergic conjunctivitis
  • Conjunctival cells
  • HLA-DR
  • Interleukin-4
  • Respiratory syncytial virus

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

Respiratory syncytial virus-induced interleukin-4 production by human conjunctival epithelial cells contributes to allergy : Preliminary study. / Fujishima, Hiroshi; Saito, Ichiro; Okamoto, Yoshitaka; Takeuchi, Tsutomu; Tsubota, Kazuo.

In: Current Eye Research, Vol. 17, No. 6, 1998, p. 656-662.

Research output: Contribution to journalArticle

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N2 - Purpose. We previously reported allergic conjunctivitis was associated with a local pattern of cytokines and identified respiratory syncytial virus (RSV) in the conjunctival epithelium, in patients with allergic conjunctivitis. We hypothesized that RSV infected the normal conjunctival epithelium and produced interleukin-4 (IL-4), causing an allergic reaction. To determine this event, we studied production of IL-4 by human conjunctival epithelial cells, after infection with RSV. Methods. Conjunctival samples were collected with a special brush and were challenged with 5 x 106 pfu/ml of live RSV. RSV infection was detected by immunocytochemical study and RT-PCR. HLA-DR antigen was expressed by flow cytometry. IL-4 was assayed by a sandwich ELISA. Results. Immunocytochemical study and RT-PCR showed that RSV infected the conjunctival epithelial cells. HLA-DR was demonstrated in the RSV-infected conjunctival epithelial cells. Average concentrations of IL-4 in culture supernatants in infected cells were 3.8 ± 1.8, 3.6 ± 1.1, and 5.4 ± 1.8 pg/ml at 24, 48, and 72 h, respectively. Levels in non-infected cells were 0.33 ± 0.0, 0.59 ± 0.69, and 0.4 ± 0.22 pg/ml, respectively (p > 0.001). Conclusion. Findings suggest that the expression of IL-4 is associated with intracellular infection by RSV in conjunctiva. With our former reports, RSV infection in conjunctival epithelial cells can be suggested as one of the possible environmental pathogens of allergic conjunctivitis.

AB - Purpose. We previously reported allergic conjunctivitis was associated with a local pattern of cytokines and identified respiratory syncytial virus (RSV) in the conjunctival epithelium, in patients with allergic conjunctivitis. We hypothesized that RSV infected the normal conjunctival epithelium and produced interleukin-4 (IL-4), causing an allergic reaction. To determine this event, we studied production of IL-4 by human conjunctival epithelial cells, after infection with RSV. Methods. Conjunctival samples were collected with a special brush and were challenged with 5 x 106 pfu/ml of live RSV. RSV infection was detected by immunocytochemical study and RT-PCR. HLA-DR antigen was expressed by flow cytometry. IL-4 was assayed by a sandwich ELISA. Results. Immunocytochemical study and RT-PCR showed that RSV infected the conjunctival epithelial cells. HLA-DR was demonstrated in the RSV-infected conjunctival epithelial cells. Average concentrations of IL-4 in culture supernatants in infected cells were 3.8 ± 1.8, 3.6 ± 1.1, and 5.4 ± 1.8 pg/ml at 24, 48, and 72 h, respectively. Levels in non-infected cells were 0.33 ± 0.0, 0.59 ± 0.69, and 0.4 ± 0.22 pg/ml, respectively (p > 0.001). Conclusion. Findings suggest that the expression of IL-4 is associated with intracellular infection by RSV in conjunctiva. With our former reports, RSV infection in conjunctival epithelial cells can be suggested as one of the possible environmental pathogens of allergic conjunctivitis.

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