Response of intra-acinar pulmonary microvessels to hypoxia, hypercapnic acidosis, and isocapnic acidosis

Kazuhiro Yamaguchi, Koichi Suzuki, Katsuhiko Naoki, Kazumi Nishio, Nagato Sato, Kei Takeshita, Hiroyasu Kudo, Takuya Aoki, Yukio Suzuki, Atsushi Miyata, Harukuni Tsumura

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

To elucidate the differential reactivity of pulmonary microvessels in the acini to hypoxia, excessive CO2, and increased H+, we investigated changes in the diameter of precapillary arterioles, postcapillary venules, and capillaries in isolated rat lungs on exposure to normocapnic hypoxia (2% O2), normoxic hypercapnia (15% CO2), and isocapnic acidosis (0.01 mol/L HCl). Microvascular diameters were precisely examined using a real-time confocal laser scanning luminescence microscope coupled to a high-sensitivity camera with an image intensifier. Measurements were made under conditions with and without indomethacin or N(ω)-nitro-L-arginine methyl ester to assess the importance of vasoactive substances produced by cyclooxygenase (COX) or NO synthase (NOS) as it relates to the reactivity of pulmonary microvessels to physiological stimuli. We found that acute hypoxia contracted precapillary arterioles that had diameters of 20 to 30 μm but did not constrict postcapillary venules of similar size. COX- and NOS-related vasoactive substances did not modulate hypoxia-elicited arteriolar constriction. Hypercapnia induced a distinct venular dilatation closely associated with vasodilators produced by COX but not by NOS. Arterioles were appreciably constricted in isocapnic acidosis when NOS, but not COX, was suppressed, whereas venules showed no constrictive response even when both enzymes were inhibited. Capillaries were neither constricted nor dilated under any experimental conditions. These findings suggest that reactivity to hypoxia, CO2, and H+ is not qualitatively similar among intra-acinar microvessels, in which COX- and NOS-associated vasoactive substances function differently.

Original languageEnglish
Pages (from-to)722-728
Number of pages7
JournalCirculation Research
Volume82
Issue number6
Publication statusPublished - 1998 Apr 6

Fingerprint

Prostaglandin-Endoperoxide Synthases
Acidosis
Microvessels
Nitric Oxide Synthase
Venules
Arterioles
Lung
Hypercapnia
Luminescence
Vasodilator Agents
Constriction
Indomethacin
Dilatation
Lasers
Hypoxia
Enzymes

Keywords

  • Acidosis
  • Acinus
  • Cyclooxygenase
  • Hypoxia
  • Nitric oxide synthase

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Yamaguchi, K., Suzuki, K., Naoki, K., Nishio, K., Sato, N., Takeshita, K., ... Tsumura, H. (1998). Response of intra-acinar pulmonary microvessels to hypoxia, hypercapnic acidosis, and isocapnic acidosis. Circulation Research, 82(6), 722-728.

Response of intra-acinar pulmonary microvessels to hypoxia, hypercapnic acidosis, and isocapnic acidosis. / Yamaguchi, Kazuhiro; Suzuki, Koichi; Naoki, Katsuhiko; Nishio, Kazumi; Sato, Nagato; Takeshita, Kei; Kudo, Hiroyasu; Aoki, Takuya; Suzuki, Yukio; Miyata, Atsushi; Tsumura, Harukuni.

In: Circulation Research, Vol. 82, No. 6, 06.04.1998, p. 722-728.

Research output: Contribution to journalArticle

Yamaguchi, K, Suzuki, K, Naoki, K, Nishio, K, Sato, N, Takeshita, K, Kudo, H, Aoki, T, Suzuki, Y, Miyata, A & Tsumura, H 1998, 'Response of intra-acinar pulmonary microvessels to hypoxia, hypercapnic acidosis, and isocapnic acidosis', Circulation Research, vol. 82, no. 6, pp. 722-728.
Yamaguchi K, Suzuki K, Naoki K, Nishio K, Sato N, Takeshita K et al. Response of intra-acinar pulmonary microvessels to hypoxia, hypercapnic acidosis, and isocapnic acidosis. Circulation Research. 1998 Apr 6;82(6):722-728.
Yamaguchi, Kazuhiro ; Suzuki, Koichi ; Naoki, Katsuhiko ; Nishio, Kazumi ; Sato, Nagato ; Takeshita, Kei ; Kudo, Hiroyasu ; Aoki, Takuya ; Suzuki, Yukio ; Miyata, Atsushi ; Tsumura, Harukuni. / Response of intra-acinar pulmonary microvessels to hypoxia, hypercapnic acidosis, and isocapnic acidosis. In: Circulation Research. 1998 ; Vol. 82, No. 6. pp. 722-728.
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