Restoration of E-cadherin expression by selective Cox-2 inhibition and the clinical relevance of the epithelial-to-mesenchymal transition in head and neck squamous cell carcinoma

Ryoichi Fujii, Yorihisa Imanishi, Katsushi Shibata, Nobuya Sakai, Koji Sakamoto, Seiji Shigetomi, Noboru Habu, Kuninori Otsuka, Yoichiro Sato, Yoshihiro Watanabe, Hiroyuki Ozawa, Toshiki Tomita, Kaori Kameyama, Masato Fujii, Kaoru Ogawa

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Background: The epithelial-to-mesenchymal transition (EMT) accompanied by the downregulation of E-cadherin has been thought to promote metastasis. Cyclooxygenase-2 (Cox-2) is presumed to contribute to cancer progression through its multifaceted function, and recently its inverse relationship with E-cadherin was suggested. The aim of the present study was to investigate whether selective Cox-2 inhibitors restore the expression of E-cadherin in head and neck squamous cell carcinoma (HNSCC) cells, and to examine the possible correlations of the expression levels of EMT-related molecules with clinicopathological factors in HNSCC. Methods. We used quantitative real-time PCR to examine the effects of three selective Cox-2 inhibitors, i.e., celecoxib, NS-398, and SC-791 on the gene expressions of E-cadherin (CDH-1) and its transcriptional repressors (SIP1, Snail, Twist) in the human HNSCC cell lines HSC-2 and HSC-4. To evaluate the changes in E-cadherin expression on the cell surface, we used a flowcytometer and immunofluorescent staining in addition to Western blotting. We evaluated and statistically analyzed the clinicopathological factors and mRNA expressions of Cox-2, CDH-1 and its repressors in surgical specimens of 40 patients with tongue squamous cell carcinoma (TSCC). Results: The selective Cox-2 inhibitors upregulated the E-cadherin expression on the cell surface of the HNSCC cells through the downregulation of its transcriptional repressors. The extent of this effect depended on the baseline expression levels of both E-cadherin and Cox-2 in each cell line. A univariate analysis showed that higher Cox-2 mRNA expression (p = 0.037), lower CDH-1 mRNA expression (p = 0.020), and advanced T-classification (p = 0.036) were significantly correlated with lymph node metastasis in TSCC. A multivariate logistic regression revealed that lower CDH-1 mRNA expression was the independent risk factor affecting lymph node metastasis (p = 0.041). Conclusions: These findings suggest that the appropriately selective administration of certain Cox-2 inhibitors may have an anti-metastatic effect through suppression of the EMT by restoring E-cadherin expression. In addition, the downregulation of CDH-1 resulting from the EMT may be closely involved in lymph node metastasis in TSCC.

Original languageEnglish
Article number40
JournalJournal of Experimental and Clinical Cancer Research
Volume33
Issue number1
DOIs
Publication statusPublished - 2014 May 10

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Epithelial-Mesenchymal Transition
Cadherins
Cyclooxygenase 2
Cyclooxygenase 2 Inhibitors
Tongue
Neoplasm Metastasis
Squamous Cell Carcinoma
Messenger RNA
Down-Regulation
Lymph Nodes
Celecoxib
Carcinoma, squamous cell of head and neck
Cell Line
Real-Time Polymerase Chain Reaction
Logistic Models
Western Blotting
Staining and Labeling
Gene Expression

Keywords

  • Cox-2 inhibition
  • E-cadherin
  • Epithelial-to-mesenchymal transition (EMT)
  • Head and neck squamous cell carcinoma (HNSCC)
  • Lymph node metastasis

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Restoration of E-cadherin expression by selective Cox-2 inhibition and the clinical relevance of the epithelial-to-mesenchymal transition in head and neck squamous cell carcinoma. / Fujii, Ryoichi; Imanishi, Yorihisa; Shibata, Katsushi; Sakai, Nobuya; Sakamoto, Koji; Shigetomi, Seiji; Habu, Noboru; Otsuka, Kuninori; Sato, Yoichiro; Watanabe, Yoshihiro; Ozawa, Hiroyuki; Tomita, Toshiki; Kameyama, Kaori; Fujii, Masato; Ogawa, Kaoru.

In: Journal of Experimental and Clinical Cancer Research, Vol. 33, No. 1, 40, 10.05.2014.

Research output: Contribution to journalArticle

Fujii, Ryoichi ; Imanishi, Yorihisa ; Shibata, Katsushi ; Sakai, Nobuya ; Sakamoto, Koji ; Shigetomi, Seiji ; Habu, Noboru ; Otsuka, Kuninori ; Sato, Yoichiro ; Watanabe, Yoshihiro ; Ozawa, Hiroyuki ; Tomita, Toshiki ; Kameyama, Kaori ; Fujii, Masato ; Ogawa, Kaoru. / Restoration of E-cadherin expression by selective Cox-2 inhibition and the clinical relevance of the epithelial-to-mesenchymal transition in head and neck squamous cell carcinoma. In: Journal of Experimental and Clinical Cancer Research. 2014 ; Vol. 33, No. 1.
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AU - Fujii, Ryoichi

AU - Imanishi, Yorihisa

AU - Shibata, Katsushi

AU - Sakai, Nobuya

AU - Sakamoto, Koji

AU - Shigetomi, Seiji

AU - Habu, Noboru

AU - Otsuka, Kuninori

AU - Sato, Yoichiro

AU - Watanabe, Yoshihiro

AU - Ozawa, Hiroyuki

AU - Tomita, Toshiki

AU - Kameyama, Kaori

AU - Fujii, Masato

AU - Ogawa, Kaoru

PY - 2014/5/10

Y1 - 2014/5/10

N2 - Background: The epithelial-to-mesenchymal transition (EMT) accompanied by the downregulation of E-cadherin has been thought to promote metastasis. Cyclooxygenase-2 (Cox-2) is presumed to contribute to cancer progression through its multifaceted function, and recently its inverse relationship with E-cadherin was suggested. The aim of the present study was to investigate whether selective Cox-2 inhibitors restore the expression of E-cadherin in head and neck squamous cell carcinoma (HNSCC) cells, and to examine the possible correlations of the expression levels of EMT-related molecules with clinicopathological factors in HNSCC. Methods. We used quantitative real-time PCR to examine the effects of three selective Cox-2 inhibitors, i.e., celecoxib, NS-398, and SC-791 on the gene expressions of E-cadherin (CDH-1) and its transcriptional repressors (SIP1, Snail, Twist) in the human HNSCC cell lines HSC-2 and HSC-4. To evaluate the changes in E-cadherin expression on the cell surface, we used a flowcytometer and immunofluorescent staining in addition to Western blotting. We evaluated and statistically analyzed the clinicopathological factors and mRNA expressions of Cox-2, CDH-1 and its repressors in surgical specimens of 40 patients with tongue squamous cell carcinoma (TSCC). Results: The selective Cox-2 inhibitors upregulated the E-cadherin expression on the cell surface of the HNSCC cells through the downregulation of its transcriptional repressors. The extent of this effect depended on the baseline expression levels of both E-cadherin and Cox-2 in each cell line. A univariate analysis showed that higher Cox-2 mRNA expression (p = 0.037), lower CDH-1 mRNA expression (p = 0.020), and advanced T-classification (p = 0.036) were significantly correlated with lymph node metastasis in TSCC. A multivariate logistic regression revealed that lower CDH-1 mRNA expression was the independent risk factor affecting lymph node metastasis (p = 0.041). Conclusions: These findings suggest that the appropriately selective administration of certain Cox-2 inhibitors may have an anti-metastatic effect through suppression of the EMT by restoring E-cadherin expression. In addition, the downregulation of CDH-1 resulting from the EMT may be closely involved in lymph node metastasis in TSCC.

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KW - Head and neck squamous cell carcinoma (HNSCC)

KW - Lymph node metastasis

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