Restriction on conjugational transfer of pLS20 in Bacillus subtilis 168

Naoto Ohtani, Mitsuru Sato, Masaru Tomita, Mitsuhiro Itaya

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Conjugational transfer of pLS20 in Bacillus subtilis Marburg 168 is restricted by the BsuM restriction-modification system. Restriction efficiency was measured using pLS20 derivatives possessing various numbers of XhoI sites, which are known to be recognized by BsuM. An increase in XhoI sites clearly reduced the conjugational efficiency of pLS20 as compared with that of pUB110 plasmid lacking XhoI.

Original languageEnglish
Pages (from-to)2472-2475
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume72
Issue number9
DOIs
Publication statusPublished - 2008

Fingerprint

DNA Restriction-Modification Enzymes
Bacilli
Bacillus subtilis
Plasmids
Derivatives

Keywords

  • Bacillus subtilis
  • BsuM
  • Conjugational transfer
  • Restriction modification

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Analytical Chemistry
  • Organic Chemistry

Cite this

Restriction on conjugational transfer of pLS20 in Bacillus subtilis 168. / Ohtani, Naoto; Sato, Mitsuru; Tomita, Masaru; Itaya, Mitsuhiro.

In: Bioscience, Biotechnology and Biochemistry, Vol. 72, No. 9, 2008, p. 2472-2475.

Research output: Contribution to journalArticle

Ohtani, Naoto ; Sato, Mitsuru ; Tomita, Masaru ; Itaya, Mitsuhiro. / Restriction on conjugational transfer of pLS20 in Bacillus subtilis 168. In: Bioscience, Biotechnology and Biochemistry. 2008 ; Vol. 72, No. 9. pp. 2472-2475.
@article{427b131ca26b4744932412ba36f932de,
title = "Restriction on conjugational transfer of pLS20 in Bacillus subtilis 168",
abstract = "Conjugational transfer of pLS20 in Bacillus subtilis Marburg 168 is restricted by the BsuM restriction-modification system. Restriction efficiency was measured using pLS20 derivatives possessing various numbers of XhoI sites, which are known to be recognized by BsuM. An increase in XhoI sites clearly reduced the conjugational efficiency of pLS20 as compared with that of pUB110 plasmid lacking XhoI.",
keywords = "Bacillus subtilis, BsuM, Conjugational transfer, Restriction modification",
author = "Naoto Ohtani and Mitsuru Sato and Masaru Tomita and Mitsuhiro Itaya",
year = "2008",
doi = "10.1271/bbb.80315",
language = "English",
volume = "72",
pages = "2472--2475",
journal = "Bioscience, Biotechnology and Biochemistry",
issn = "0916-8451",
publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",
number = "9",

}

TY - JOUR

T1 - Restriction on conjugational transfer of pLS20 in Bacillus subtilis 168

AU - Ohtani, Naoto

AU - Sato, Mitsuru

AU - Tomita, Masaru

AU - Itaya, Mitsuhiro

PY - 2008

Y1 - 2008

N2 - Conjugational transfer of pLS20 in Bacillus subtilis Marburg 168 is restricted by the BsuM restriction-modification system. Restriction efficiency was measured using pLS20 derivatives possessing various numbers of XhoI sites, which are known to be recognized by BsuM. An increase in XhoI sites clearly reduced the conjugational efficiency of pLS20 as compared with that of pUB110 plasmid lacking XhoI.

AB - Conjugational transfer of pLS20 in Bacillus subtilis Marburg 168 is restricted by the BsuM restriction-modification system. Restriction efficiency was measured using pLS20 derivatives possessing various numbers of XhoI sites, which are known to be recognized by BsuM. An increase in XhoI sites clearly reduced the conjugational efficiency of pLS20 as compared with that of pUB110 plasmid lacking XhoI.

KW - Bacillus subtilis

KW - BsuM

KW - Conjugational transfer

KW - Restriction modification

UR - http://www.scopus.com/inward/record.url?scp=52649109286&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=52649109286&partnerID=8YFLogxK

U2 - 10.1271/bbb.80315

DO - 10.1271/bbb.80315

M3 - Article

VL - 72

SP - 2472

EP - 2475

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

SN - 0916-8451

IS - 9

ER -