Abstract
Objective: We sought to examine the effect of resveratrol (3,4',5-trihydroxy-trans-stilbene), a plant-derived polyphenolic compound, on the development of abdominal aortic aneurysm (AAA). Methods: AAA was induced in mice by periaortic application of CaCl 2. NaCl (0.9%)-applied mice were used as a sham group. Mice were treated with intraperitoneal injection of PBS (Sham/CON, AAA/CON, n=30 for each) or resveratrol (100mg/kg/day) (AAA/RSVT, n=30). Six weeks after the operation, aortic tissue was excised for further examinations. Results: Aortic diameter was enlarged in AAA/CON compared with Sham/CON. Resveratrol treatment reduced the aneurysm size and inflammatory cell infiltration in the aortic wall compared with AAA/CON. Elastica Van Gieson staining showed destruction of the wavy morphology of the elastic lamellae in AAA/CON, while it was preserved in AAA/RSVT. The increased mRNA expression of monocyte chemotactic protein-1, tumor necrosis factor-α, intercellular adhesion molecule-1, CD68, vascular endothelial growth factor-A, p47, glutathione peroxidase (GPX)1 and GPX3 were attenuated by resveratrol treatment (all p<0.05). Administration of resveratrol decreased protein expression of phospho-p65 in AAA. The increased 8-hydroxy-2'-deoxyguanosine-positive cell count and 4-hydroxy-2-nonenal-positive cell count in AAA were also reduced by resveratrol treatment. Zymographic activity of matrix metalloproteinase (MMP)-9 and MMP-2 was lower in AAA/RSVT compared with AAA/CON (both p<0.05). Compared with AAA/CON, Mac-2 + macrophages and CD31 + vessels in the aortic wall were decreased in AAA/RSVT (both p<0.05). Conclusion: Treatment with resveratrol in mice prevented the development of CaCl 2-induced AAA, in association with reduced inflammation, oxidative stress, neoangiogenesis, and extracellular matrix disruption. These findings suggest therapeutic potential of resveratrol for AAA.
| Original language | English |
|---|---|
| Pages (from-to) | 350-357 |
| Number of pages | 8 |
| Journal | Atherosclerosis |
| Volume | 217 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2011 Aug |
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Keywords
- Abdominal aortic aneurysm
- Extracellular matrix
- Inflammation
- Neoangiogenesis
- Oxidative stress
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine
Cite this
Resveratrol prevents the development of abdominal aortic aneurysm through attenuation of inflammation, oxidative stress, and neovascularization. / Kaneko, Hidehiro; Anzai, Toshihisa; Morisawa, Maho; Kohno, Takashi; Nagai, Toshiyuki; Anzai, Atsushi; Takahashi, Toshiyuki; Shimoda, Masayuki; Sasaki, Aya; Maekawa, Yuichiro; Yoshimura, Koichi; Aoki, Hiroki; Tsubota, Kazuo; Yoshikawa, Tsutomu; Okada, Yasunori; Ogawa, Satoshi; Fukuda, Keiichi.
In: Atherosclerosis, Vol. 217, No. 2, 08.2011, p. 350-357.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Resveratrol prevents the development of abdominal aortic aneurysm through attenuation of inflammation, oxidative stress, and neovascularization
AU - Kaneko, Hidehiro
AU - Anzai, Toshihisa
AU - Morisawa, Maho
AU - Kohno, Takashi
AU - Nagai, Toshiyuki
AU - Anzai, Atsushi
AU - Takahashi, Toshiyuki
AU - Shimoda, Masayuki
AU - Sasaki, Aya
AU - Maekawa, Yuichiro
AU - Yoshimura, Koichi
AU - Aoki, Hiroki
AU - Tsubota, Kazuo
AU - Yoshikawa, Tsutomu
AU - Okada, Yasunori
AU - Ogawa, Satoshi
AU - Fukuda, Keiichi
PY - 2011/8
Y1 - 2011/8
N2 - Objective: We sought to examine the effect of resveratrol (3,4',5-trihydroxy-trans-stilbene), a plant-derived polyphenolic compound, on the development of abdominal aortic aneurysm (AAA). Methods: AAA was induced in mice by periaortic application of CaCl 2. NaCl (0.9%)-applied mice were used as a sham group. Mice were treated with intraperitoneal injection of PBS (Sham/CON, AAA/CON, n=30 for each) or resveratrol (100mg/kg/day) (AAA/RSVT, n=30). Six weeks after the operation, aortic tissue was excised for further examinations. Results: Aortic diameter was enlarged in AAA/CON compared with Sham/CON. Resveratrol treatment reduced the aneurysm size and inflammatory cell infiltration in the aortic wall compared with AAA/CON. Elastica Van Gieson staining showed destruction of the wavy morphology of the elastic lamellae in AAA/CON, while it was preserved in AAA/RSVT. The increased mRNA expression of monocyte chemotactic protein-1, tumor necrosis factor-α, intercellular adhesion molecule-1, CD68, vascular endothelial growth factor-A, p47, glutathione peroxidase (GPX)1 and GPX3 were attenuated by resveratrol treatment (all p<0.05). Administration of resveratrol decreased protein expression of phospho-p65 in AAA. The increased 8-hydroxy-2'-deoxyguanosine-positive cell count and 4-hydroxy-2-nonenal-positive cell count in AAA were also reduced by resveratrol treatment. Zymographic activity of matrix metalloproteinase (MMP)-9 and MMP-2 was lower in AAA/RSVT compared with AAA/CON (both p<0.05). Compared with AAA/CON, Mac-2 + macrophages and CD31 + vessels in the aortic wall were decreased in AAA/RSVT (both p<0.05). Conclusion: Treatment with resveratrol in mice prevented the development of CaCl 2-induced AAA, in association with reduced inflammation, oxidative stress, neoangiogenesis, and extracellular matrix disruption. These findings suggest therapeutic potential of resveratrol for AAA.
AB - Objective: We sought to examine the effect of resveratrol (3,4',5-trihydroxy-trans-stilbene), a plant-derived polyphenolic compound, on the development of abdominal aortic aneurysm (AAA). Methods: AAA was induced in mice by periaortic application of CaCl 2. NaCl (0.9%)-applied mice were used as a sham group. Mice were treated with intraperitoneal injection of PBS (Sham/CON, AAA/CON, n=30 for each) or resveratrol (100mg/kg/day) (AAA/RSVT, n=30). Six weeks after the operation, aortic tissue was excised for further examinations. Results: Aortic diameter was enlarged in AAA/CON compared with Sham/CON. Resveratrol treatment reduced the aneurysm size and inflammatory cell infiltration in the aortic wall compared with AAA/CON. Elastica Van Gieson staining showed destruction of the wavy morphology of the elastic lamellae in AAA/CON, while it was preserved in AAA/RSVT. The increased mRNA expression of monocyte chemotactic protein-1, tumor necrosis factor-α, intercellular adhesion molecule-1, CD68, vascular endothelial growth factor-A, p47, glutathione peroxidase (GPX)1 and GPX3 were attenuated by resveratrol treatment (all p<0.05). Administration of resveratrol decreased protein expression of phospho-p65 in AAA. The increased 8-hydroxy-2'-deoxyguanosine-positive cell count and 4-hydroxy-2-nonenal-positive cell count in AAA were also reduced by resveratrol treatment. Zymographic activity of matrix metalloproteinase (MMP)-9 and MMP-2 was lower in AAA/RSVT compared with AAA/CON (both p<0.05). Compared with AAA/CON, Mac-2 + macrophages and CD31 + vessels in the aortic wall were decreased in AAA/RSVT (both p<0.05). Conclusion: Treatment with resveratrol in mice prevented the development of CaCl 2-induced AAA, in association with reduced inflammation, oxidative stress, neoangiogenesis, and extracellular matrix disruption. These findings suggest therapeutic potential of resveratrol for AAA.
KW - Abdominal aortic aneurysm
KW - Extracellular matrix
KW - Inflammation
KW - Neoangiogenesis
KW - Oxidative stress
UR - http://www.scopus.com/inward/record.url?scp=79960698213&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79960698213&partnerID=8YFLogxK
U2 - 10.1016/j.atherosclerosis.2011.03.042
DO - 10.1016/j.atherosclerosis.2011.03.042
M3 - Article
C2 - 21530968
AN - SCOPUS:79960698213
VL - 217
SP - 350
EP - 357
JO - Atherosclerosis
JF - Atherosclerosis
SN - 0021-9150
IS - 2
ER -