Retinal-specific ATP-binding cassette transporter (ABCR/ABCA4) is expressed at the choroid plexus in rat brain

Jiraganya Bhongsatiern, Sumio Ohtsuki, Masanori Tachikawa, Satoko Hori, Tetsuya Terasaki

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

ATP-binding cassette (ABC) transporter A4 is a member of the ABC transporter subfamily A which has been reported to be exclusively expressed in the retina. In contrast, a previous report has suggested a possible relationship between ABCA4 and CNS function. The purpose of the present study was to investigate the localization of ABCA4 mRNA and protein in rat brain. In situ hybridization analysis revealed that ABCA4 mRNA was localized in the lateral ventricles. RT-PCR analysis detected ABCA4 mRNA in isolated rat choroid plexus and conditionally immortalized rat choroid plexus epithelial cells (TR-CSFB). Furthermore, ABCA4 protein was also detected in the isolated rat choroid plexus at about 250 KDa by western blot analysis, and its apparent molecular size was reduced by N-glycosidase F treatment. These results suggest that glycosylated ABCA4 protein is expressed in rat choroid plexus epithelial cells. ABCA4 may play a role in the function of the blood-cerebrospinal fluid barrier and affect CSF conditions.

Original languageEnglish
Pages (from-to)1277-1280
Number of pages4
JournalJournal of Neurochemistry
Volume92
Issue number5
DOIs
Publication statusPublished - 2005 Mar

Keywords

  • ATP-binding cassette transporter A4
  • Blood-cerebrospinal fluid barrier
  • Choroid plexus
  • Epithelial cell

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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