Retinoic acid-driven Hox1 is required in the epidermis for forming the otic/atrial placodes during ascidian metamorphosis

Yasunori Sasakura, Miyuki Kanda, Taku Ikeda, Takeo Horie, Narudo Kawai, Yosuke Ogura, Reiko Yoshida, Akiko Hozumi, Nori Satoh, Shigeki Fujiwara

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Retinoic acid (RA)-mediated expression of the homeobox gene Hox1 is a hallmark of the chordate central nervous system (CNS). It has been suggested that the RA-Hox1 network also functions in the epidermal ectoderm of chordates. Here, we show that in the urochordate ascidian Ciona intestinalis, RA-Hox1 in the epidermal ectoderm is necessary for formation of the atrial siphon placode (ASP), a structure homologous to the vertebrate otic placode. Loss of Hox1 function resulted in loss of the ASP, which could be rescued by expressing Hox1 in the epidermis. As previous studies showed that RA directly upregulates Hox1 in the epidermis of Ciona larvae, we also examined the role of RA in ASP formation. We showed that abolishment of RA resulted in loss of the ASP, which could be rescued by forced expression of Hox1 in the epidermis. Our results suggest that RA-Hox1 in the epidermal ectoderm played a key role in the acquisition of the otic placode during chordate evolution.

Original languageEnglish
Pages (from-to)2156-2160
Number of pages5
JournalDevelopment (Cambridge)
Volume139
Issue number12
DOIs
Publication statusPublished - 2012 Jun 15
Externally publishedYes

Fingerprint

Urochordata
Tretinoin
Epidermis
Ear
Chordata
Ectoderm
Ciona intestinalis
Homeobox Genes
Larva
Vertebrates
Up-Regulation
Central Nervous System

Keywords

  • Ascidian
  • Atrial siphon
  • Ciona intestinalis
  • Hox1
  • Placode
  • Retinoic acid

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology

Cite this

Retinoic acid-driven Hox1 is required in the epidermis for forming the otic/atrial placodes during ascidian metamorphosis. / Sasakura, Yasunori; Kanda, Miyuki; Ikeda, Taku; Horie, Takeo; Kawai, Narudo; Ogura, Yosuke; Yoshida, Reiko; Hozumi, Akiko; Satoh, Nori; Fujiwara, Shigeki.

In: Development (Cambridge), Vol. 139, No. 12, 15.06.2012, p. 2156-2160.

Research output: Contribution to journalArticle

Sasakura, Y, Kanda, M, Ikeda, T, Horie, T, Kawai, N, Ogura, Y, Yoshida, R, Hozumi, A, Satoh, N & Fujiwara, S 2012, 'Retinoic acid-driven Hox1 is required in the epidermis for forming the otic/atrial placodes during ascidian metamorphosis', Development (Cambridge), vol. 139, no. 12, pp. 2156-2160. https://doi.org/10.1242/dev.080234
Sasakura, Yasunori ; Kanda, Miyuki ; Ikeda, Taku ; Horie, Takeo ; Kawai, Narudo ; Ogura, Yosuke ; Yoshida, Reiko ; Hozumi, Akiko ; Satoh, Nori ; Fujiwara, Shigeki. / Retinoic acid-driven Hox1 is required in the epidermis for forming the otic/atrial placodes during ascidian metamorphosis. In: Development (Cambridge). 2012 ; Vol. 139, No. 12. pp. 2156-2160.
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