Retinoic acid-induced gene expression of tissue transglutaminase via protein kinase c-dependent pathway in mouse peritoneal macrophages

Isao Ishii, Michio Ui

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Culture of mouse resident peritoneal macrophages with retinoic acid resulted in increased expression of the tissue transglutaminase gene as revealed by increases in the maximal velocity of the enzyme reaction in the cytosol and in the enzyme mRNA level. Protein kinase C-activating phorbol esters and okadaic acid, both of which were without effect on the enzyme induction by themselves, enhanced the retinoic acid-induced gene expression, which was in turn inhibited partially by pertussis toxin and totally by inhibitors of protein kinase C in either the presence or absence of phorbol esters. Retinoic acid was more effective in the "conditioned" medium, in which macrophages had been cultured for a time longer than 4 h, than in the "fresh" medium. The retinoic acid induction of transglutaminase was accompanied by increased phosphatidylinositol turnover and phosphatidic acid generation, which were efficiently suppressed by prior exposure of cells to pertussis toxin. It is likely that certain autocrine factor(s) liberated during culture of macrophages may afford conditions favorable for retinoic acid-induced gene expression, presumably via pertussis toxin-sensitive G protein-mediated phosphoinositide metabolism leading to activation of protein kinase C.

Original languageEnglish
Pages (from-to)1197-1202
Number of pages6
JournalJournal of Biochemistry
Volume115
Issue number6
Publication statusPublished - 1994 Jun
Externally publishedYes

Fingerprint

Protein Kinase C
Macrophage
Macrophages
Peritoneal Macrophages
Tretinoin
Gene expression
Protein Kinases
Gene Expression
Mouse
Pathway
Enzymes
Tissue
Proteins
Pertussis Toxin
Acids
Dependent
Proof by induction
G Protein
Phorbol Esters
Phosphatidylinositols

Keywords

  • Macrophage
  • Phosphoinositide metabolism
  • Protein kinase C
  • Retinoic acid
  • Transglutaminase

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Molecular Biology
  • Biochemistry

Cite this

Retinoic acid-induced gene expression of tissue transglutaminase via protein kinase c-dependent pathway in mouse peritoneal macrophages. / Ishii, Isao; Ui, Michio.

In: Journal of Biochemistry, Vol. 115, No. 6, 06.1994, p. 1197-1202.

Research output: Contribution to journalArticle

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