Retrovirus‐mediated Gene Transfer Targeted to Malignant Glioma Cells in Murine Brain

Masanobu Yamada, Keiji Shimizu, Yasuyoshi Miyao, Toru Hayakawa, Kazuhiro Ikenaka, Kensuke Nakahira, Kazunori Nakajima, Tetsushi Kagawa, Katsuhiko Mikoshiba

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17 Citations (Scopus)

Abstract

A murine model for meningeal metastasis of malignant glioma was developed to study selective gene transfer into tumor cells and to establish a reliable means of determining the rate of tumor cell infection. A murine ecotropic retroviral vector was created in which the Escherichia coliβ‐galactosidase gene served as a marker for gene expression from the integrated retrovirus. This retrovirus exhibited a high rate of infectivity in RSV‐M mouse glioma cells in vitro. The recombinant retrovirus was injected directly into the cisterna magna of the mice. Staining of β‐galactosidase showed that the rate of gene integration was high in the disseminated glioma cells. These results suggest the possibility of retrovirus‐mediated gene therapy for meningeal dissemination of malignant glioma.

Original languageEnglish
Pages (from-to)1244-1247
Number of pages4
JournalJapanese Journal of Cancer Research
Volume83
Issue number12
DOIs
Publication statusPublished - 1992 Dec
Externally publishedYes

Keywords

  • Dissemination
  • Gene transfer
  • Glioma
  • Retroviral vector

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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  • Cite this

    Yamada, M., Shimizu, K., Miyao, Y., Hayakawa, T., Ikenaka, K., Nakahira, K., Nakajima, K., Kagawa, T., & Mikoshiba, K. (1992). Retrovirus‐mediated Gene Transfer Targeted to Malignant Glioma Cells in Murine Brain. Japanese Journal of Cancer Research, 83(12), 1244-1247. https://doi.org/10.1111/j.1349-7006.1992.tb02752.x