Reversal of multidrug resistance by 7-O-Benzoylpyripyropene A in multidrug-resistant tumor cells

M. C. Rho, M. Hayashi, A. Fukami, R. Obata, T. Sunazuka, H. Tomoda, K. Komiyama, S. Omura

Research output: Contribution to journalArticle

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Abstract

7-O-Benzoylpyripyropene A (7-O-BzP), a semi-synthetic analog of pyripyropene, was investigated for its reversing effect on multidrug-resistant (MDR) tumor cells. 7-O-BzP (6.25 μg/ml) completely reversed resistance against vincristine and adriamycin in vincristine-resistant KB cells (VJ-300) and adriamycin-resistant P388 cells (P388/ADR), respectively. 7-O-BzP alone had no effect on the growth of drug sensitive and drug-resistant cells. 7-O-BzP (6.25 μg/ml) significantly enhanced accumulation of [3H]vincristine in VJ-300 cells and completely inhibited the binding of [3H]azidopine to the P-glycoprotein in VJ-300 cells and P388/ADR cells. The result suggests that 7-O-BzP effectively reverses P-glycoprotein-related MDR by interacting directly with P-glycoprotein in drug resistant VJ-300 and P388/ADR cells.

Original languageEnglish
Pages (from-to)1201-1206
Number of pages6
JournalJournal of Antibiotics
Volume53
Issue number10
Publication statusPublished - 2000
Externally publishedYes

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Multiple Drug Resistance
P-Glycoprotein
Vincristine
Neoplasms
Doxorubicin
Pharmaceutical Preparations
KB Cells
7-O-benzoylpyripyropene A
Growth

ASJC Scopus subject areas

  • Pharmacology
  • Molecular Medicine

Cite this

Rho, M. C., Hayashi, M., Fukami, A., Obata, R., Sunazuka, T., Tomoda, H., ... Omura, S. (2000). Reversal of multidrug resistance by 7-O-Benzoylpyripyropene A in multidrug-resistant tumor cells. Journal of Antibiotics, 53(10), 1201-1206.

Reversal of multidrug resistance by 7-O-Benzoylpyripyropene A in multidrug-resistant tumor cells. / Rho, M. C.; Hayashi, M.; Fukami, A.; Obata, R.; Sunazuka, T.; Tomoda, H.; Komiyama, K.; Omura, S.

In: Journal of Antibiotics, Vol. 53, No. 10, 2000, p. 1201-1206.

Research output: Contribution to journalArticle

Rho, MC, Hayashi, M, Fukami, A, Obata, R, Sunazuka, T, Tomoda, H, Komiyama, K & Omura, S 2000, 'Reversal of multidrug resistance by 7-O-Benzoylpyripyropene A in multidrug-resistant tumor cells', Journal of Antibiotics, vol. 53, no. 10, pp. 1201-1206.
Rho MC, Hayashi M, Fukami A, Obata R, Sunazuka T, Tomoda H et al. Reversal of multidrug resistance by 7-O-Benzoylpyripyropene A in multidrug-resistant tumor cells. Journal of Antibiotics. 2000;53(10):1201-1206.
Rho, M. C. ; Hayashi, M. ; Fukami, A. ; Obata, R. ; Sunazuka, T. ; Tomoda, H. ; Komiyama, K. ; Omura, S. / Reversal of multidrug resistance by 7-O-Benzoylpyripyropene A in multidrug-resistant tumor cells. In: Journal of Antibiotics. 2000 ; Vol. 53, No. 10. pp. 1201-1206.
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