Reversed-phase high-performance liquid chromatography of iron(II) and copper(II) chelates with 4,7-diphenyl-1,10-phenanthroline disulfonate

X. Mudasir, Meiko Arai, Naoki Yoshioka, Hidenari Inoue

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The chromatographic behaviour of negatively-charged iron(II) and copper(II) chelates with 4,7-diphenyl-1,10-phenanthroline (bathophenanthroline) disulfonate (BPS) on a reversed-phase Inertsil ODS-2 column was studied by using methanol-water (60:40, v/v) containing 0.02 M tetramethylammonium bromide (TMAB) and 0.025 M tris(hydroxymethyl)aminomethane (Tris)-HCl at pH 7.5 as a mobile phase. The possibility that the hydrophilic Tris-HCl buffer also serves as a counter ion together with TMAB was briefly discussed. Chromatographic parameters influencing the separation of the chelates were optimized. The method was successfully applied to indirectly determine iron(II) and iron(III) contents in natural water. Nickel(II) ion and possibly organic pollutants interfere with the present method.

Original languageEnglish
Pages (from-to)171-176
Number of pages6
JournalJournal of Chromatography A
Volume799
Issue number1-2
DOIs
Publication statusPublished - 1998 Mar 13

Fingerprint

Iron Chelating Agents
High performance liquid chromatography
Reverse-Phase Chromatography
Copper
Iron
High Pressure Liquid Chromatography
Tromethamine
Ions
Radiation counters
Water
Organic pollutants
Nickel
Methanol
Buffers
bathophenanthroline

Keywords

  • 4,7-Diphenyl-1,10-phenanthroline disulfonate
  • Copper
  • Iron
  • Metal chelates

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Reversed-phase high-performance liquid chromatography of iron(II) and copper(II) chelates with 4,7-diphenyl-1,10-phenanthroline disulfonate. / Mudasir, X.; Arai, Meiko; Yoshioka, Naoki; Inoue, Hidenari.

In: Journal of Chromatography A, Vol. 799, No. 1-2, 13.03.1998, p. 171-176.

Research output: Contribution to journalArticle

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AU - Inoue, Hidenari

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AB - The chromatographic behaviour of negatively-charged iron(II) and copper(II) chelates with 4,7-diphenyl-1,10-phenanthroline (bathophenanthroline) disulfonate (BPS) on a reversed-phase Inertsil ODS-2 column was studied by using methanol-water (60:40, v/v) containing 0.02 M tetramethylammonium bromide (TMAB) and 0.025 M tris(hydroxymethyl)aminomethane (Tris)-HCl at pH 7.5 as a mobile phase. The possibility that the hydrophilic Tris-HCl buffer also serves as a counter ion together with TMAB was briefly discussed. Chromatographic parameters influencing the separation of the chelates were optimized. The method was successfully applied to indirectly determine iron(II) and iron(III) contents in natural water. Nickel(II) ion and possibly organic pollutants interfere with the present method.

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