TY - JOUR
T1 - Reversible off-on fluorescence probe for hypoxia and imaging of hypoxia-normoxia cycles in live cells
AU - Takahashi, Shodai
AU - Piao, Wen
AU - Matsumura, Yuriko
AU - Komatsu, Toru
AU - Ueno, Tasuku
AU - Terai, Takuya
AU - Kamachi, Toshiaki
AU - Kohno, Masahiro
AU - Nagano, Tetsuo
AU - Hanaoka, Kenjiro
PY - 2012/12/5
Y1 - 2012/12/5
N2 - We report a fully reversible off-on fluorescence probe for hypoxia. The design employs QSY-21 as a Förster resonance energy transfer (FRET) acceptor and cyanine dye Cy5 as a FRET donor, based on our finding that QSY-21 undergoes one-electron bioreduction to the radical under hypoxia, with an absorbance decrease at 660 nm. At that point, FRET can no longer occur, and the dye becomes strongly fluorescent. Upon recovery of normoxia, the radical is immediately reoxidized to QSY-21, with loss of fluorescence due to restoration of FRET. We show that this probe, RHyCy5, can monitor repeated hypoxia-normoxia cycles in live cells.
AB - We report a fully reversible off-on fluorescence probe for hypoxia. The design employs QSY-21 as a Förster resonance energy transfer (FRET) acceptor and cyanine dye Cy5 as a FRET donor, based on our finding that QSY-21 undergoes one-electron bioreduction to the radical under hypoxia, with an absorbance decrease at 660 nm. At that point, FRET can no longer occur, and the dye becomes strongly fluorescent. Upon recovery of normoxia, the radical is immediately reoxidized to QSY-21, with loss of fluorescence due to restoration of FRET. We show that this probe, RHyCy5, can monitor repeated hypoxia-normoxia cycles in live cells.
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U2 - 10.1021/ja310049d
DO - 10.1021/ja310049d
M3 - Article
C2 - 23157219
AN - SCOPUS:84870673782
SN - 0002-7863
VL - 134
SP - 19588
EP - 19591
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
IS - 48
ER -