Reversible whole-organism cell cycle arrest in a living vertebrate

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

In vivo cell cycle analysis in higher eukaryotes has been limited by the challenge of preserving the integrity of the living organism while visualizing dividing cells. Japanese medaka in order to visualize and manipulate the cell cycle progression in a live vertebrate. Our stable transgenic histone H2B-GFP medaka line allows fluorescence-based monitoring of the chromosomes. The system has a high specificity, with a strong GFP signal labeling the chromatin architecture. The subcellular resolution ensures detection of both normal and abnormal divisions in live recordings. This translates into the possibility to quantify temporal and spatial aspects of the cell cycle, such as length or nuclear size, as well as to expose drug toxicity at the earliest stage. We also show that acclimation to cold, a prominent feature of the eurytherm medaka, is a valuable natural way of inducing a reversible cell cycle arrest in the entire living organism. Our results suggest that this manipulation can be performed from the early stages of development, has no toxicity and does not alter the cell cycle profile of the embryo.

Original languageEnglish
Pages (from-to)620-627
Number of pages8
JournalCell Cycle
Volume8
Issue number4
Publication statusPublished - 2009 Feb 15

Fingerprint

Oryzias
Cell Cycle Checkpoints
Vertebrates
Cell Cycle
Acclimatization
Drug-Related Side Effects and Adverse Reactions
Eukaryota
Histones
Chromatin
Embryonic Structures
Chromosomes
Fluorescence

Keywords

  • Cold treatment
  • Histone H2B
  • In vivo cell cycle
  • Reversible arrest
  • Transgenic medaka

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Developmental Biology

Cite this

Reversible whole-organism cell cycle arrest in a living vertebrate. / Sampetrean, Oltea; Lida, Shin Ichi; Makino, Shinji; Matsuzaki, Yuriko; Ohno, Kikuo; Saya, Hideyuki.

In: Cell Cycle, Vol. 8, No. 4, 15.02.2009, p. 620-627.

Research output: Contribution to journalArticle

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