Rex1/Zfp42 is dispensable for pluripotency in mouse ES cells

Shinji Masui; Satoshi Ohtsuka; Rika Yagi; Kadue Takahashi; Minoru S.H. Ko; Hitoshi Niwa

doi:10.1186/1471-213X-8-45

Rex1/Zfp42 is dispensable for pluripotency in mouse ES cells

Shinji Masui, Satoshi Ohtsuka, Rika Yagi, Kadue Takahashi, Minoru S.H. Ko, Hitoshi Niwa

Research output: Contribution to journal › Article › peer-review

75 Citations (Scopus)

Abstract

Background. Rex1/Zfp42 has been extensively used as a marker for the undifferentiated state of pluripotent stem cells. However, its function in pluripotent stem cells including embryonic stem (ES) cells remained unclear although its involvement in visceral endoderm differentiation in F9 embryonal carcinoma (EC) cells was reported. Results. We showed the function of Rex1 in mouse ES cells as well as in embryos using the conventional gene targeting strategy. Our results clearly indicated that Rex1 function is dispensable for both the maintenance of pluripotency in ES cells and the development of embryos. However, Rex1-/- ES cells showed the defect to induce a subset of the marker genes of visceral endoderm, when differentiated as embryoid body, as found in EC cells. Conclusion. Rex1 should be regarded just as a marker of pluripotency without functional significance like the activity of alkaline phosphatase.

Original language	English
Article number	45
Journal	BMC Developmental Biology
Volume	8
DOIs	https://doi.org/10.1186/1471-213X-8-45
Publication status	Published - 2008
Externally published	Yes

ASJC Scopus subject areas

Developmental Biology

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10.1186/1471-213X-8-45

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title = "Rex1/Zfp42 is dispensable for pluripotency in mouse ES cells",

abstract = "Background. Rex1/Zfp42 has been extensively used as a marker for the undifferentiated state of pluripotent stem cells. However, its function in pluripotent stem cells including embryonic stem (ES) cells remained unclear although its involvement in visceral endoderm differentiation in F9 embryonal carcinoma (EC) cells was reported. Results. We showed the function of Rex1 in mouse ES cells as well as in embryos using the conventional gene targeting strategy. Our results clearly indicated that Rex1 function is dispensable for both the maintenance of pluripotency in ES cells and the development of embryos. However, Rex1-/- ES cells showed the defect to induce a subset of the marker genes of visceral endoderm, when differentiated as embryoid body, as found in EC cells. Conclusion. Rex1 should be regarded just as a marker of pluripotency without functional significance like the activity of alkaline phosphatase.",

author = "Shinji Masui and Satoshi Ohtsuka and Rika Yagi and Kadue Takahashi and Ko, {Minoru S.H.} and Hitoshi Niwa",

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T1 - Rex1/Zfp42 is dispensable for pluripotency in mouse ES cells

AU - Masui, Shinji

AU - Ohtsuka, Satoshi

AU - Yagi, Rika

AU - Takahashi, Kadue

AU - Ko, Minoru S.H.

AU - Niwa, Hitoshi

PY - 2008

Y1 - 2008

N2 - Background. Rex1/Zfp42 has been extensively used as a marker for the undifferentiated state of pluripotent stem cells. However, its function in pluripotent stem cells including embryonic stem (ES) cells remained unclear although its involvement in visceral endoderm differentiation in F9 embryonal carcinoma (EC) cells was reported. Results. We showed the function of Rex1 in mouse ES cells as well as in embryos using the conventional gene targeting strategy. Our results clearly indicated that Rex1 function is dispensable for both the maintenance of pluripotency in ES cells and the development of embryos. However, Rex1-/- ES cells showed the defect to induce a subset of the marker genes of visceral endoderm, when differentiated as embryoid body, as found in EC cells. Conclusion. Rex1 should be regarded just as a marker of pluripotency without functional significance like the activity of alkaline phosphatase.

AB - Background. Rex1/Zfp42 has been extensively used as a marker for the undifferentiated state of pluripotent stem cells. However, its function in pluripotent stem cells including embryonic stem (ES) cells remained unclear although its involvement in visceral endoderm differentiation in F9 embryonal carcinoma (EC) cells was reported. Results. We showed the function of Rex1 in mouse ES cells as well as in embryos using the conventional gene targeting strategy. Our results clearly indicated that Rex1 function is dispensable for both the maintenance of pluripotency in ES cells and the development of embryos. However, Rex1-/- ES cells showed the defect to induce a subset of the marker genes of visceral endoderm, when differentiated as embryoid body, as found in EC cells. Conclusion. Rex1 should be regarded just as a marker of pluripotency without functional significance like the activity of alkaline phosphatase.

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