TY - JOUR
T1 - Rex1/Zfp42 is dispensable for pluripotency in mouse ES cells
AU - Masui, Shinji
AU - Ohtsuka, Satoshi
AU - Yagi, Rika
AU - Takahashi, Kadue
AU - Ko, Minoru S.H.
AU - Niwa, Hitoshi
N1 - Funding Information:
We thank Ryo Matoba and Yulan Piao for microarray hybridization. This work was supported by a RIKEN grant, grants for the 21st Century COE program, and grant-in-aid for scientific research from the Ministry of Education, Culture, Sports, Science and Technology of Japan, and the funding from the CREST program of the Japan Science and Technology Agency to H.N. The work was also supported in part by the Intramural Research Program of the National Institute of Aging, National Institutes of Health.
PY - 2008
Y1 - 2008
N2 - Background. Rex1/Zfp42 has been extensively used as a marker for the undifferentiated state of pluripotent stem cells. However, its function in pluripotent stem cells including embryonic stem (ES) cells remained unclear although its involvement in visceral endoderm differentiation in F9 embryonal carcinoma (EC) cells was reported. Results. We showed the function of Rex1 in mouse ES cells as well as in embryos using the conventional gene targeting strategy. Our results clearly indicated that Rex1 function is dispensable for both the maintenance of pluripotency in ES cells and the development of embryos. However, Rex1-/- ES cells showed the defect to induce a subset of the marker genes of visceral endoderm, when differentiated as embryoid body, as found in EC cells. Conclusion. Rex1 should be regarded just as a marker of pluripotency without functional significance like the activity of alkaline phosphatase.
AB - Background. Rex1/Zfp42 has been extensively used as a marker for the undifferentiated state of pluripotent stem cells. However, its function in pluripotent stem cells including embryonic stem (ES) cells remained unclear although its involvement in visceral endoderm differentiation in F9 embryonal carcinoma (EC) cells was reported. Results. We showed the function of Rex1 in mouse ES cells as well as in embryos using the conventional gene targeting strategy. Our results clearly indicated that Rex1 function is dispensable for both the maintenance of pluripotency in ES cells and the development of embryos. However, Rex1-/- ES cells showed the defect to induce a subset of the marker genes of visceral endoderm, when differentiated as embryoid body, as found in EC cells. Conclusion. Rex1 should be regarded just as a marker of pluripotency without functional significance like the activity of alkaline phosphatase.
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U2 - 10.1186/1471-213X-8-45
DO - 10.1186/1471-213X-8-45
M3 - Article
C2 - 18433507
AN - SCOPUS:44049089423
VL - 8
JO - BMC Developmental Biology
JF - BMC Developmental Biology
SN - 1471-213X
M1 - 45
ER -