Rex1/Zfp42 is dispensable for pluripotency in mouse ES cells

Shinji Masui, Satoshi Ohtsuka, Rika Yagi, Kadue Takahashi, Minoru Ko, Hitoshi Niwa

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Background. Rex1/Zfp42 has been extensively used as a marker for the undifferentiated state of pluripotent stem cells. However, its function in pluripotent stem cells including embryonic stem (ES) cells remained unclear although its involvement in visceral endoderm differentiation in F9 embryonal carcinoma (EC) cells was reported. Results. We showed the function of Rex1 in mouse ES cells as well as in embryos using the conventional gene targeting strategy. Our results clearly indicated that Rex1 function is dispensable for both the maintenance of pluripotency in ES cells and the development of embryos. However, Rex1-/- ES cells showed the defect to induce a subset of the marker genes of visceral endoderm, when differentiated as embryoid body, as found in EC cells. Conclusion. Rex1 should be regarded just as a marker of pluripotency without functional significance like the activity of alkaline phosphatase.

Original languageEnglish
Article number45
JournalBMC Developmental Biology
Volume8
DOIs
Publication statusPublished - 2008
Externally publishedYes

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Embryonic Stem Cells
Embryonal Carcinoma Stem Cells
Pluripotent Stem Cells
Endoderm
Embryoid Bodies
Gene Targeting
Embryonic Development
Alkaline Phosphatase
Embryonic Structures
Maintenance
Genes
Mouse Embryonic Stem Cells

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Rex1/Zfp42 is dispensable for pluripotency in mouse ES cells. / Masui, Shinji; Ohtsuka, Satoshi; Yagi, Rika; Takahashi, Kadue; Ko, Minoru; Niwa, Hitoshi.

In: BMC Developmental Biology, Vol. 8, 45, 2008.

Research output: Contribution to journalArticle

Masui, Shinji ; Ohtsuka, Satoshi ; Yagi, Rika ; Takahashi, Kadue ; Ko, Minoru ; Niwa, Hitoshi. / Rex1/Zfp42 is dispensable for pluripotency in mouse ES cells. In: BMC Developmental Biology. 2008 ; Vol. 8.
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AU - Ko, Minoru

AU - Niwa, Hitoshi

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