RMD-1, a novel microtubule-associated protein, functions in chromosome segregation in Caenorhabditis elegans

Kumiko Oishi, Hideyuki Okano, Hitoshi Sawa

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

For proper chromosome segregation, the sister kinetochores must attach to microtubules extending from the opposite spindle poles. Any errors in microtubule attachment can induce aneuploidy. In this study, we identify a novel conserved Caenorhabditis elegans micro tubule-associated protein, regulator of microtubule dynamics 1 (RMD-1), that localizes to spindle microtubules and spindle poles. Depletion of RMD-1 induces severe defects in chromosome segregation, probably through merotelic attachments between microtubules and chromosomes. Although rmd-1 embryos also have a mild defect in microtubule growth, we find that mutants of the microtubule growth regulator XMAP215/ZYG-9 show much weaker segregation defects. This suggests that the microtubule growth defect in rmd-1 embryos does not cause abnormal chromosome segregation. We also see that RMD-1 interacts with aurora B in vitro. Our results suggest that RMD-1 functions in chromosome segregation in C. elegans embryos, possibly through the aurora B-mediated pathway. Human homologues of RMD-1 could also bind microtubules, which would suggest a function for these proteins in chromosome segregation during mitosis in other organisms as well.

Original languageEnglish
Pages (from-to)1149-1162
Number of pages14
JournalJournal of Cell Biology
Volume179
Issue number6
DOIs
Publication statusPublished - 2007 Dec 17

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Chromosome Segregation
Microtubule-Associated Proteins
Caenorhabditis elegans
Microtubules
Spindle Poles
Embryonic Structures
Growth
Kinetochores
Aneuploidy
Mitosis
Chromosomes

ASJC Scopus subject areas

  • Cell Biology

Cite this

RMD-1, a novel microtubule-associated protein, functions in chromosome segregation in Caenorhabditis elegans. / Oishi, Kumiko; Okano, Hideyuki; Sawa, Hitoshi.

In: Journal of Cell Biology, Vol. 179, No. 6, 17.12.2007, p. 1149-1162.

Research output: Contribution to journalArticle

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