RNA elimination machinery targeting meiotic mRNAs promotes facultative heterochromatin formation

Martin Zofall, Soichiro Yamanaka, Francisca E. Reyes-Turcu, Ke Zhang, Chanan Rubin, Shiv I S Grewal

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

Facultative heterochromatin that changes during cellular differentiation coordinates regulated gene expression, but its assembly is poorly understood. Here, we describe facultative heterochromatin islands in fission yeast and show that their formation at meiotic genes requires factors that eliminate meiotic messenger RNAs (mRNAs) during vegetative growth. Blocking production of meiotic mRNA or loss of RNA elimination factors, including Mmi1 and Red1 proteins, abolishes heterochromatin islands. RNA elimination machinery is enriched at meiotic loci and interacts with Clr4/SUV39h, a methyltransferase involved in heterochromatin assembly. Heterochromatin islands disassemble in response to nutritional signals that induce sexual differentiation. This process involves the antisilencing factor Epe1, the loss of which causes dramatic increase in heterochromatic loci. Our analyses uncover unexpected regulatory roles for mRNA-processing factors that assemble dynamic heterochromatin to modulate gene expression.

Original languageEnglish
Pages (from-to)96-100
Number of pages5
JournalScience
Volume335
Issue number6064
DOIs
Publication statusPublished - 2012 Jan 6
Externally publishedYes

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Heterochromatin
RNA
Messenger RNA
Gene Expression
Sex Differentiation
Schizosaccharomyces
Methyltransferases
Growth
Genes
Proteins

ASJC Scopus subject areas

  • General

Cite this

Zofall, M., Yamanaka, S., Reyes-Turcu, F. E., Zhang, K., Rubin, C., & Grewal, S. I. S. (2012). RNA elimination machinery targeting meiotic mRNAs promotes facultative heterochromatin formation. Science, 335(6064), 96-100. https://doi.org/10.1126/science.1211651

RNA elimination machinery targeting meiotic mRNAs promotes facultative heterochromatin formation. / Zofall, Martin; Yamanaka, Soichiro; Reyes-Turcu, Francisca E.; Zhang, Ke; Rubin, Chanan; Grewal, Shiv I S.

In: Science, Vol. 335, No. 6064, 06.01.2012, p. 96-100.

Research output: Contribution to journalArticle

Zofall, M, Yamanaka, S, Reyes-Turcu, FE, Zhang, K, Rubin, C & Grewal, SIS 2012, 'RNA elimination machinery targeting meiotic mRNAs promotes facultative heterochromatin formation', Science, vol. 335, no. 6064, pp. 96-100. https://doi.org/10.1126/science.1211651
Zofall M, Yamanaka S, Reyes-Turcu FE, Zhang K, Rubin C, Grewal SIS. RNA elimination machinery targeting meiotic mRNAs promotes facultative heterochromatin formation. Science. 2012 Jan 6;335(6064):96-100. https://doi.org/10.1126/science.1211651
Zofall, Martin ; Yamanaka, Soichiro ; Reyes-Turcu, Francisca E. ; Zhang, Ke ; Rubin, Chanan ; Grewal, Shiv I S. / RNA elimination machinery targeting meiotic mRNAs promotes facultative heterochromatin formation. In: Science. 2012 ; Vol. 335, No. 6064. pp. 96-100.
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