RNA Interference: A New Mechanism by which FMRP Acts in the Normal Brain? What Can Drosophila Teach Us?

Haruhiko Siomi, Akira Ishizuka, Mikiko C. Siomi

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Fragile X syndrome is the most common heritable form of mental retardation caused by loss-of-function mutations in the FMR1 gene. The FMR1 gene encodes an RNA-binding protein that associates with translating ribosomes and acts as a negative translational regulator. Recent work in Drosophila melanogaster has shown that the fly homolog of FMR1 (dFMR1) plays an important role in regulating neuronal morphology, which may underlie the observed deficits in behaviors of dFMR1 mutant flies. Biochemical analysis has revealed that dFMR1 forms a complex that includes ribosomal proteins and, surprisingly, Argonaute2 (AGO2), an essential component of the RNA-induced silencing complex (RISC) that mediates RNA interference (RNAi) in Drosophila. dFMR1 also associates with Dicer, another essential processing enzyme of the RNAi pathway. Moreover, both a micro-RNA (miRNA) and short interfering RNAs (siRNAs) can coimmunoprecipitate with dFMR1. Together these findings suggest that dFMR1 functions in an RNAi-related apparatus to regulate the expression of its target genes at the level of translation. These findings raise the possibility that Fragile X syndrome may be the result of a protein synthesis abnormality caused by a defect in an RNAi-related apparatus. Because the core mechanisms of complex behaviors such as learning and memory and circadian rhythms appear to be conserved, studies of Fragile X syndrome using Drosophila as a model provide an economy-of-scale for identifying biological processes that likely underlie the abnormal morphology of dendritic spines and behavioral disturbances observed in Fragile X patients.

Original languageEnglish
Pages (from-to)68-74
Number of pages7
JournalMental Retardation and Developmental Disabilities Research Reviews
Volume10
Issue number1
DOIs
Publication statusPublished - 2004
Externally publishedYes

Fingerprint

RNA Interference
Fragile X Syndrome
Drosophila
Brain
Diptera
RNA-Induced Silencing Complex
Genes
Biological Phenomena
Dendritic Spines
RNA-Binding Proteins
Ribosomal Proteins
Circadian Rhythm
Drosophila melanogaster
MicroRNAs
Ribosomes
Intellectual Disability
Small Interfering RNA
Learning
Mutation
Enzymes

Keywords

  • Drosophila
  • FMR1
  • miRNA
  • RNAi
  • Translational repression

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Neuropsychology and Physiological Psychology
  • Genetics(clinical)

Cite this

RNA Interference : A New Mechanism by which FMRP Acts in the Normal Brain? What Can Drosophila Teach Us? / Siomi, Haruhiko; Ishizuka, Akira; Siomi, Mikiko C.

In: Mental Retardation and Developmental Disabilities Research Reviews, Vol. 10, No. 1, 2004, p. 68-74.

Research output: Contribution to journalArticle

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