RNAi and overexpression of genes in ovarian somatic cells

Kuniaki Saito

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Emerging evidence indicates that PIWI proteins, in collaboration with PIWI-interacting RNAs (piRNAs), play a critical role in retrotransposon silencing in Drosophila gonadal somatic and germ-line cells. The recent establishment of female germ-line stem cells/ovarian somatic sheet and its derivative cell line, ovarian somatic cells (OSCs), allows researchers to study the molecular functions of several protein factors involved in the primary piRNA pathway in Drosophila. Although transgene expression is difficult to achieve in gonad-derived cell lines, transfection of both expression vectors and knockdown reagents is highly effective in OSCs. Here, I focus on techniques that knockdown or overexpress genes of interest in OSCs.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages25-33
Number of pages9
Volume1093
DOIs
Publication statusPublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1093
ISSN (Print)10643745

Fingerprint

RNA Interference
Germ Cells
Drosophila
Genes
Cell Line
Retroelements
Gonads
Transgenes
Small Interfering RNA
Transfection
Proteins
Stem Cells
Research Personnel
RNA

Keywords

  • Cell line
  • Drosophila
  • OSC
  • Overexpression
  • piRNA
  • Retrotransposon
  • RNAi

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Saito, K. (2014). RNAi and overexpression of genes in ovarian somatic cells. In Methods in Molecular Biology (Vol. 1093, pp. 25-33). (Methods in Molecular Biology; Vol. 1093). https://doi.org/10.1007/978-1-62703-694-8-3

RNAi and overexpression of genes in ovarian somatic cells. / Saito, Kuniaki.

Methods in Molecular Biology. Vol. 1093 2014. p. 25-33 (Methods in Molecular Biology; Vol. 1093).

Research output: Chapter in Book/Report/Conference proceedingChapter

Saito, K 2014, RNAi and overexpression of genes in ovarian somatic cells. in Methods in Molecular Biology. vol. 1093, Methods in Molecular Biology, vol. 1093, pp. 25-33. https://doi.org/10.1007/978-1-62703-694-8-3
Saito K. RNAi and overexpression of genes in ovarian somatic cells. In Methods in Molecular Biology. Vol. 1093. 2014. p. 25-33. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-62703-694-8-3
Saito, Kuniaki. / RNAi and overexpression of genes in ovarian somatic cells. Methods in Molecular Biology. Vol. 1093 2014. pp. 25-33 (Methods in Molecular Biology).
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