RNAi and overexpression of genes in ovarian somatic cells

Kuniaki Saito

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Citations (Scopus)

Abstract

Emerging evidence indicates that PIWI proteins, in collaboration with PIWI-interacting RNAs (piRNAs), play a critical role in retrotransposon silencing in Drosophila gonadal somatic and germ-line cells. The recent establishment of female germ-line stem cells/ovarian somatic sheet and its derivative cell line, ovarian somatic cells (OSCs), allows researchers to study the molecular functions of several protein factors involved in the primary piRNA pathway in Drosophila. Although transgene expression is difficult to achieve in gonad-derived cell lines, transfection of both expression vectors and knockdown reagents is highly effective in OSCs. Here, I focus on techniques that knockdown or overexpress genes of interest in OSCs.

Original languageEnglish
Title of host publicationPIWI-Interacting RNAs
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages25-33
Number of pages9
ISBN (Print)9781627036931
DOIs
Publication statusPublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1093
ISSN (Print)1064-3745

Keywords

  • Cell line
  • Drosophila
  • OSC
  • Overexpression
  • RNAi
  • Retrotransposon
  • piRNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Saito, K. (2014). RNAi and overexpression of genes in ovarian somatic cells. In PIWI-Interacting RNAs: Methods and Protocols (pp. 25-33). (Methods in Molecular Biology; Vol. 1093). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-694-8_3