RNAi in living mice.

Hidetoshi Hasuwa, Masaru Okabe

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

By introducing double-stranded RNAs (dsRNAs), it was shown that mRNAs that share sequences are destroyed, and that the translation step is severely downregulated (RNAi). This technique was demonstrated to be a very powerful tool for reverse genetics in Caenorhabditis elegans. However, studies have shown that RNAi can be achieved in living mice. In this chapter, we used "green mice" and "green rats" as model animals to demonstrate silencing of green fluorescent protein (GFP) expression by RNAi. In order to silence the gene, we produced transgenic mouse lines that produce dsRNA that is driven by the H1 promoter. It was demonstrated that the transgenically expressed, double stranded RNA could silence the GFP throughout the body of the mouse or rat and throughout the lifetime.

Original languageEnglish
Pages (from-to)501-508
Number of pages8
JournalMethods in molecular biology (Clifton, N.J.)
Volume252
Publication statusPublished - 2004
Externally publishedYes

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RNA Interference
Double-Stranded RNA
Green Fluorescent Proteins
Reverse Genetics
Caenorhabditis elegans
Transgenic Mice
Down-Regulation
Animal Models
Messenger RNA
Genes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

RNAi in living mice. / Hasuwa, Hidetoshi; Okabe, Masaru.

In: Methods in molecular biology (Clifton, N.J.), Vol. 252, 2004, p. 501-508.

Research output: Contribution to journalArticle

Hasuwa, Hidetoshi ; Okabe, Masaru. / RNAi in living mice. In: Methods in molecular biology (Clifton, N.J.). 2004 ; Vol. 252. pp. 501-508.
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