Abstract
Genome editing technology greatly facilitates the genetic modification of various cells and animals. The common marmoset (Callithrix jacchus), a small non-human primate which exhibits high reproductive efficiency, is a widely used animal model in biomedical research. Developing genome editing techniques in the common marmoset will further enhance its utility. Here, we report the successful establishment of a knock-in (KI) method for marmoset embryonic stem cells (ESCs), which is based on the CRISPR-Cas9 system. The use of CRISPR-Cas9, mediated by homologous recombination (HR), enhanced the KI efficiency in marmoset ESCs. Furthermore, we succeeded in performing KI in early-stage marmoset embryos. In the course of the experiments, we found that HR in the marmoset ESCs is innately highly efficient. This suggested that the marmoset possesses a repair mechanism for DNA double-strand breaks. The current study will facilitate the generation of genetically modified marmosets and gene function analysis in the marmoset.
| Original language | English |
|---|---|
| Article number | 1528 |
| Journal | Scientific Reports |
| Volume | 9 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2019 Dec 1 |
Fingerprint
ASJC Scopus subject areas
- General
Cite this
Robust and efficient knock-in in embryonic stem cells and early-stage embryos of the common marmoset using the CRISPR-Cas9 system. / Yoshimatsu, Sho; Okahara, Junko; Sone, Takefumi; Takeda, Yuta; Nakamura, Mari; Sasaki, Erika; Kishi, Noriyuki; Shiozawa, Seiji; Okano, Hideyuki.
In: Scientific Reports, Vol. 9, No. 1, 1528, 01.12.2019.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Robust and efficient knock-in in embryonic stem cells and early-stage embryos of the common marmoset using the CRISPR-Cas9 system
AU - Yoshimatsu, Sho
AU - Okahara, Junko
AU - Sone, Takefumi
AU - Takeda, Yuta
AU - Nakamura, Mari
AU - Sasaki, Erika
AU - Kishi, Noriyuki
AU - Shiozawa, Seiji
AU - Okano, Hideyuki
PY - 2019/12/1
Y1 - 2019/12/1
N2 - Genome editing technology greatly facilitates the genetic modification of various cells and animals. The common marmoset (Callithrix jacchus), a small non-human primate which exhibits high reproductive efficiency, is a widely used animal model in biomedical research. Developing genome editing techniques in the common marmoset will further enhance its utility. Here, we report the successful establishment of a knock-in (KI) method for marmoset embryonic stem cells (ESCs), which is based on the CRISPR-Cas9 system. The use of CRISPR-Cas9, mediated by homologous recombination (HR), enhanced the KI efficiency in marmoset ESCs. Furthermore, we succeeded in performing KI in early-stage marmoset embryos. In the course of the experiments, we found that HR in the marmoset ESCs is innately highly efficient. This suggested that the marmoset possesses a repair mechanism for DNA double-strand breaks. The current study will facilitate the generation of genetically modified marmosets and gene function analysis in the marmoset.
AB - Genome editing technology greatly facilitates the genetic modification of various cells and animals. The common marmoset (Callithrix jacchus), a small non-human primate which exhibits high reproductive efficiency, is a widely used animal model in biomedical research. Developing genome editing techniques in the common marmoset will further enhance its utility. Here, we report the successful establishment of a knock-in (KI) method for marmoset embryonic stem cells (ESCs), which is based on the CRISPR-Cas9 system. The use of CRISPR-Cas9, mediated by homologous recombination (HR), enhanced the KI efficiency in marmoset ESCs. Furthermore, we succeeded in performing KI in early-stage marmoset embryos. In the course of the experiments, we found that HR in the marmoset ESCs is innately highly efficient. This suggested that the marmoset possesses a repair mechanism for DNA double-strand breaks. The current study will facilitate the generation of genetically modified marmosets and gene function analysis in the marmoset.
UR - http://www.scopus.com/inward/record.url?scp=85061156432&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85061156432&partnerID=8YFLogxK
U2 - 10.1038/s41598-018-37990-w
DO - 10.1038/s41598-018-37990-w
M3 - Article
C2 - 30728412
AN - SCOPUS:85061156432
VL - 9
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
IS - 1
M1 - 1528
ER -