Robust production of human neural cells by establishing neuroepithelial-like stem cells from peripheral blood mononuclear cell-derived feeder-free iPSCs under xeno-free conditions

Miho Isoda, Jun Kohyama, Akio Iwanami, Tsukasa Sanosaka, Keiko Sugai, Ryo Yamaguchi, Takuya Matsumoto, Masaya Nakamura, Hideyuki Okano

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Neural stem/progenitor cells (NS/PCs) derived from human induced pluripotent stem cells (hiPSCs) are expected to be a valuable cell source for cell therapies that target central nervous system disorders. For clinical applications, NS/PCs should be induced and maintained under clinical grade conditions, which are challenging to achieve. In the present study, we established a procedure to obtain xeno-free long-term self-renewing neuroepithelial-like stem cells (xf-lt-NES cells) from feeder-free hiPSCs using a newly developed xeno-free medium, StemFit®AS200. xf-lt-NES cells were cultured for long periods in StemFit®AS200 while retaining normal karyotypes, NS/PC marker expression and differentiation capacity for neuronal and glial differentiation in vitro and in vivo. Furthermore, the cells were cryopreserved using a defined serum-free freezing reagent, which demonstrated the feasibility of this xeno-free culture system for large-scale lt-NES cell production and cell banking. Taken together, our system represents a promising approach for the manufacture of clinically relevant products for cell therapy using NS/PCs.

Original languageEnglish
JournalNeuroscience Research
DOIs
Publication statusAccepted/In press - 2016 Mar 19

Fingerprint

Neuroepithelial Cells
Neural Stem Cells
Blood Cells
Stem Cells
Induced Pluripotent Stem Cells
Cell- and Tissue-Based Therapy
Feeder Cells
Central Nervous System Diseases
Differentiation Antigens
Karyotype
Neuroglia
Freezing
Cultured Cells
Peripheral Blood Stem Cells
Serum

Keywords

  • Long-term self-renewing neuroepithelial-like stem cells (lt-NES cells)
  • Neural induction
  • Peripheral blood mononuclear cell-derived feeder-free human iPSCs
  • Regenerative medicine
  • Xeno-free

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

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title = "Robust production of human neural cells by establishing neuroepithelial-like stem cells from peripheral blood mononuclear cell-derived feeder-free iPSCs under xeno-free conditions",
abstract = "Neural stem/progenitor cells (NS/PCs) derived from human induced pluripotent stem cells (hiPSCs) are expected to be a valuable cell source for cell therapies that target central nervous system disorders. For clinical applications, NS/PCs should be induced and maintained under clinical grade conditions, which are challenging to achieve. In the present study, we established a procedure to obtain xeno-free long-term self-renewing neuroepithelial-like stem cells (xf-lt-NES cells) from feeder-free hiPSCs using a newly developed xeno-free medium, StemFit{\circledR}AS200. xf-lt-NES cells were cultured for long periods in StemFit{\circledR}AS200 while retaining normal karyotypes, NS/PC marker expression and differentiation capacity for neuronal and glial differentiation in vitro and in vivo. Furthermore, the cells were cryopreserved using a defined serum-free freezing reagent, which demonstrated the feasibility of this xeno-free culture system for large-scale lt-NES cell production and cell banking. Taken together, our system represents a promising approach for the manufacture of clinically relevant products for cell therapy using NS/PCs.",
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AU - Isoda, Miho

AU - Kohyama, Jun

AU - Iwanami, Akio

AU - Sanosaka, Tsukasa

AU - Sugai, Keiko

AU - Yamaguchi, Ryo

AU - Matsumoto, Takuya

AU - Nakamura, Masaya

AU - Okano, Hideyuki

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