Role of adhesion molecules in an animal model of endotoxin-induced acute lung injury

A. Ishizaka, Kenzo Soejima, S. Tasaka, M. Kanazawa

Research output: Contribution to journalArticle

Abstract

We studied the role of adhesion molecules in acute lung injury caused by lipolysaccharide (LPS). Forty-eight female guinea pigs were divided into three groups: saline (n = 12); B464, an LPS antagonist, (0.2 mg/kg i.v.) (n = 12); LPS (0.02 mg/kg i.v.) (n = 12); and LPS + B464 (n = 12). The numbers of polymorphonuclear cells (PMN) in blood sampled over 4 hours were counted. Accumulation of PMNs in the lungs was determined by counting the number of PMNs in lung-tissue samples fixed for light microscopic examination. The lung wet-to-dry weight ratio and the 125I-albumin tissue-to-plasma ratio were used to assess lung injury. Human umbilical-vein endothelial cells were treated with B464 and then stimulated with either LPS or tumor necrosis factor. Expression of ICAM-1 and ELAM-1 was estimated by enzyme-linked immunosorbent assay. After LPS injection, light microscopy revealed decreases in peripheral PMN counts, and accumulation of PMNs in the lungs. Increases in the two indices of lung injury were also observed. These changes were attenuated by prior treatment with B464. The LPS-induced increases in ICAM-1 and ELAM-1 expression were dose-dependently suppressed by B464. These results suggest that pulmonary accumulation of activated PMNs plays an important role in LPS-induced lung injury.

Original languageEnglish
Pages (from-to)136-140
Number of pages5
JournalJapanese Journal of Thoracic Diseases
Volume34
Issue numberSUPPL.
Publication statusPublished - 1996

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Acute Lung Injury
Endotoxins
Animal Models
Lung Injury
Lung
E-Selectin
Intercellular Adhesion Molecule-1
Cell Count
Light
Human Umbilical Vein Endothelial Cells
Pulmonary Edema
Microscopy
Albumins
Blood Cells
Guinea Pigs
Tumor Necrosis Factor-alpha
Enzyme-Linked Immunosorbent Assay
B 464
Weights and Measures
Injections

Keywords

  • Acute lung injury
  • Adhesion molecule
  • Capillary permeability
  • Endotoxin
  • Pulmonary edema

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Role of adhesion molecules in an animal model of endotoxin-induced acute lung injury. / Ishizaka, A.; Soejima, Kenzo; Tasaka, S.; Kanazawa, M.

In: Japanese Journal of Thoracic Diseases, Vol. 34, No. SUPPL., 1996, p. 136-140.

Research output: Contribution to journalArticle

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