Role of alternative splicing of the rat erythropoietin receptor gene in normal and erythroleukemia cells

Masayuki Fujita, Rei Takahashi, Ping Liang, Hideyuki Saya, Faramarz Ashoori, Mitsuko Tachi, Sohei Kitazawa, Sakan Maeda

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Abstract

An alternatively splicing of the rat erythropoietin receptor (EpoR) gene was identified in normal and erythroleukemia cells by the reverse transcription PCR method. Insertion of a 105 bp fragment at the region corresponding to the extracellular domain of rat EpoR was found. The insert sequence, which encodes additional 21 amino acids, is similar to that previously found in the mouse EpoR gene, however, has an additional 27 bp direct repeat. Due to the presence of a stop codon in the insert, the alternative transcript is translated to a truncated and soluble form of EpoR which is preferentially expressed in liver, spleen, kidney, heart, and bone marrow cells as well as cultured erythroleukemia cells. These findings suggest that alternative splicing of the EpoR gene may play an important role in proliferation and differentiation of rat erythroid cells.

Original languageEnglish
Pages (from-to)444-445
Number of pages2
JournalLeukemia
Volume11
Issue numberSUPPL. 3
Publication statusPublished - 1997 Jan 1
Externally publishedYes

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ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

Cite this

Fujita, M., Takahashi, R., Liang, P., Saya, H., Ashoori, F., Tachi, M., Kitazawa, S., & Maeda, S. (1997). Role of alternative splicing of the rat erythropoietin receptor gene in normal and erythroleukemia cells. Leukemia, 11(SUPPL. 3), 444-445.