Role of CD18-ICAM-1 in the entrapment of stimulated leukocytes in alveolar capillaries of perfused rat lungs

Takuya Aoki, Yukio Suzuki, Kazumi Nishio, Kouichi Suzuki, Atsusi Miyata, Yutaka Iigou, Hiroshi Serizawa, Harukuni Tsumura, Yuzuru Ishimura, Makoto Suematsu, Kazuhiro Yamaguchi

Research output: Contribution to journalArticle

Abstract

This study aimed to examine the behavior of stimulated leukocytes in the pulmonary microcirculation. The leukocyte-endothelium interaction was visualized under physiological shear rates in perfused rat lungs using high- speed confocal laser video microscopy. Leukocytes labeled with carboxyfluorescein were stimulated with cytokine-induced neutrophil chemoattractant (CINC/gro), which caused L-selectin shedding and inverse upregulation of CD18. Neither unstimulated nor stimulated leukocytes exhibited rolling in either pulmonary arterioles or venules, whereas both were sequestered in capillaries. Approximately 50% of stimulated leukocytes showed a transient cessation of movement in pulmonary capillaries. The CINC/gro stimulation, which inhibited leukocyte rolling and adhesion to mesenteric venules, reduced leukocyte velocity and increased leukocytes in pulmonary capillaries. Pretreatment with monoclonal antibodies against intercellular adhesion molecule-1 (ICAM-1) or CD18 attenuated these changes. Confocal microfluorography revealed constitutive expression of ICAM-1 not only in venules but also abundantly in capillary networks. These results suggest that selectin-independent, CD18-ICAM-1-dependent capillary sequestration is one of the major mechanisms by which activated leukocytes accumulate in the lungs.

Original languageEnglish
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume273
Issue number5 42-5
Publication statusPublished - 1997

Fingerprint

Intercellular Adhesion Molecule-1
Leukocytes
Lung
Venules
Leukocyte Rolling
Confocal Microscopy
L-Selectin
Selectins
Video Microscopy
Chemotactic Factors
Arterioles
Microcirculation
Endothelium
Neutrophils
Up-Regulation
Monoclonal Antibodies
Cytokines

Keywords

  • L- selectin
  • Leukocyte adhesion
  • Leukocyte plugging
  • Mesenteric microcirculation
  • Pulmonary microcirculation

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Role of CD18-ICAM-1 in the entrapment of stimulated leukocytes in alveolar capillaries of perfused rat lungs. / Aoki, Takuya; Suzuki, Yukio; Nishio, Kazumi; Suzuki, Kouichi; Miyata, Atsusi; Iigou, Yutaka; Serizawa, Hiroshi; Tsumura, Harukuni; Ishimura, Yuzuru; Suematsu, Makoto; Yamaguchi, Kazuhiro.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 273, No. 5 42-5, 1997.

Research output: Contribution to journalArticle

Aoki, T, Suzuki, Y, Nishio, K, Suzuki, K, Miyata, A, Iigou, Y, Serizawa, H, Tsumura, H, Ishimura, Y, Suematsu, M & Yamaguchi, K 1997, 'Role of CD18-ICAM-1 in the entrapment of stimulated leukocytes in alveolar capillaries of perfused rat lungs', American Journal of Physiology - Heart and Circulatory Physiology, vol. 273, no. 5 42-5.
Aoki, Takuya ; Suzuki, Yukio ; Nishio, Kazumi ; Suzuki, Kouichi ; Miyata, Atsusi ; Iigou, Yutaka ; Serizawa, Hiroshi ; Tsumura, Harukuni ; Ishimura, Yuzuru ; Suematsu, Makoto ; Yamaguchi, Kazuhiro. / Role of CD18-ICAM-1 in the entrapment of stimulated leukocytes in alveolar capillaries of perfused rat lungs. In: American Journal of Physiology - Heart and Circulatory Physiology. 1997 ; Vol. 273, No. 5 42-5.
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