Role of gut cryptopatches in early extrathymic maturation of intestinal intraepithelial T cells

Takatoku Oida, Kenji Suzuki, Masanobu Nanno, Yutaka Kanamori, Hisashi Saito, Eiro Kubota, Shingo Kato, Mamoru Itoh, Shuichi Kaminogawa, Hiromichi Ishikawa

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65 Citations (Scopus)

Abstract

Lympho-hemopoietic progenitors residing in murine gut cryptopatches (CP) have been shown to generate intestinal intraepithelial T cells (IEL). To investigate the role of CP in progenitor maturation, we analyzed IEL in male mice with a truncated mutation of common cytokine receptor γ-chain (CRγ(- /Y)) in which CP were undetectable. IEL-expressing TCR-γδ (γδ-IEL) were absent, and a drastically reduced number of Thy-1(high)CD4+ and Thy- 1(high)CD8αβ+ αβ-IEL were present in CRγ(-/Y) mice, whereas these αβ- IEL disappeared from athymic CRγ(-/Y) littermate mice. Athymic CRγ(-/Y) mice possessed a small TCR- and α(E)β7 integrin-negative IEL population, characterized by the disappearance of the extrathymic CD8αα+ subset, that expressed pre-Tα, RAG-2, and TCR-Cβ but not CD3ε transcripts. These TCR- IEL from athymic CRγ(-/Y) mice did not undergo Dβ-Jβ and Vδ-Jδ joinings, despite normal rearrangements at the TCR-β and -δ loci in thymocytes from euthymic CRγ(-/Y) mice. In contrast, athymic severe combined immunodeficient mice in which CP developed normally possessed two major TCR-α(E)β7+ CD8αα+ and CD8- IEL populations that expressed pre-Tα, RAG-2, TCR-Cβ, and CD3ε transcripts. These findings underscore the role of gut CP in the early extrathymic maturation of CD8αα+ IEL, including cell-surface expression of α(E)β7 integrin, CD3ε gene transcription, and TCR gene rearrangements.

Original languageEnglish
Pages (from-to)3616-3626
Number of pages11
JournalJournal of Immunology
Volume164
Issue number7
DOIs
Publication statusPublished - 2000 Apr 1

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Oida, T., Suzuki, K., Nanno, M., Kanamori, Y., Saito, H., Kubota, E., Kato, S., Itoh, M., Kaminogawa, S., & Ishikawa, H. (2000). Role of gut cryptopatches in early extrathymic maturation of intestinal intraepithelial T cells. Journal of Immunology, 164(7), 3616-3626. https://doi.org/10.4049/jimmunol.164.7.3616