Role of gut cryptopatches in early extrathymic maturation of intestinal intraepithelial T cells

Takatoku Oida, Kenji Suzuki, Masanobu Nanno, Yutaka Kanamori, Hisashi Saito, Eiro Kubota, Shingo Kato, Mamoru Itoh, Shuichi Kaminogawa, Hiromichi Ishikawa

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Abstract

Lympho-hemopoietic progenitors residing in murine gut cryptopatches (CP) have been shown to generate intestinal intraepithelial T cells (IEL). To investigate the role of CP in progenitor maturation, we analyzed IEL in male mice with a truncated mutation of common cytokine receptor γ-chain (CRγ(- /Y)) in which CP were undetectable. IEL-expressing TCR-γδ (γδ-IEL) were absent, and a drastically reduced number of Thy-1(high)CD4+ and Thy- 1(high)CD8αβ+ αβ-IEL were present in CRγ(-/Y) mice, whereas these αβ- IEL disappeared from athymic CRγ(-/Y) littermate mice. Athymic CRγ(-/Y) mice possessed a small TCR- and α(E)β7 integrin-negative IEL population, characterized by the disappearance of the extrathymic CD8αα+ subset, that expressed pre-Tα, RAG-2, and TCR-Cβ but not CD3ε transcripts. These TCR- IEL from athymic CRγ(-/Y) mice did not undergo Dβ-Jβ and Vδ-Jδ joinings, despite normal rearrangements at the TCR-β and -δ loci in thymocytes from euthymic CRγ(-/Y) mice. In contrast, athymic severe combined immunodeficient mice in which CP developed normally possessed two major TCR-α(E)β7 + CD8αα+ and CD8- IEL populations that expressed pre-Tα, RAG-2, TCR-Cβ, and CD3ε transcripts. These findings underscore the role of gut CP in the early extrathymic maturation of CD8αα+ IEL, including cell-surface expression of α(E)β7 integrin, CD3ε gene transcription, and TCR gene rearrangements.

Original languageEnglish
Pages (from-to)3616-3626
Number of pages11
JournalJournal of Immunology
Volume164
Issue number7
Publication statusPublished - 2000 Apr 1

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T-Lymphocytes
Integrins
Cytokine Receptors
SCID Mice
Gene Rearrangement
Thymocytes
Nude Mice
Population
Mutation
Genes

ASJC Scopus subject areas

  • Immunology

Cite this

Oida, T., Suzuki, K., Nanno, M., Kanamori, Y., Saito, H., Kubota, E., ... Ishikawa, H. (2000). Role of gut cryptopatches in early extrathymic maturation of intestinal intraepithelial T cells. Journal of Immunology, 164(7), 3616-3626.

Role of gut cryptopatches in early extrathymic maturation of intestinal intraepithelial T cells. / Oida, Takatoku; Suzuki, Kenji; Nanno, Masanobu; Kanamori, Yutaka; Saito, Hisashi; Kubota, Eiro; Kato, Shingo; Itoh, Mamoru; Kaminogawa, Shuichi; Ishikawa, Hiromichi.

In: Journal of Immunology, Vol. 164, No. 7, 01.04.2000, p. 3616-3626.

Research output: Contribution to journalArticle

Oida, T, Suzuki, K, Nanno, M, Kanamori, Y, Saito, H, Kubota, E, Kato, S, Itoh, M, Kaminogawa, S & Ishikawa, H 2000, 'Role of gut cryptopatches in early extrathymic maturation of intestinal intraepithelial T cells', Journal of Immunology, vol. 164, no. 7, pp. 3616-3626.
Oida T, Suzuki K, Nanno M, Kanamori Y, Saito H, Kubota E et al. Role of gut cryptopatches in early extrathymic maturation of intestinal intraepithelial T cells. Journal of Immunology. 2000 Apr 1;164(7):3616-3626.
Oida, Takatoku ; Suzuki, Kenji ; Nanno, Masanobu ; Kanamori, Yutaka ; Saito, Hisashi ; Kubota, Eiro ; Kato, Shingo ; Itoh, Mamoru ; Kaminogawa, Shuichi ; Ishikawa, Hiromichi. / Role of gut cryptopatches in early extrathymic maturation of intestinal intraepithelial T cells. In: Journal of Immunology. 2000 ; Vol. 164, No. 7. pp. 3616-3626.
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