Abstract
Hepatic tumors including hepatocellular carcinoma were generated by carbon tetrachloride in transgenic mice carrying a human c-H-ras gene (rasH2 mice). RasH2 mice express 2 to 3 times more ras protein (ras p21) in the liver than do non-Tg mice. When carbon tetrachloride was administered, the rasH2 mice produced about 5 times as many hepatic tumors than did the non-transgenic mice. However, neither the 10-100 times higher ras p21 expression required for murine fibroblast transformation by itself nor the mutational activation of the H-ras gene was observed in carbon tetrachloride-induced hepatic tumors. These results show that H-ras proto-oncogene expression in the murine liver, even if it is not high enough to transform cells, also causes liver tumors when CCl4 are repeatedly given.
| Original language | English |
|---|---|
| Pages (from-to) | 371-379 |
| Number of pages | 9 |
| Journal | Biochemistry and Molecular Biology International |
| Volume | 42 |
| Issue number | 2 |
| Publication status | Published - 1997 Jun |
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Keywords
- H-ras
- Hepatocellular caricmona
- Neoplastic nodule
- Over expression
- Point mutation
ASJC Scopus subject areas
- Biochemistry
- Genetics
- Molecular Biology
Cite this
Role of H-ras gene in chronic liver damage in mice, by using transgenic mice carrying a human c-H-ras protogene without mutations. / Tsunematsu, Satoshi; Saito, Hidetsugu; Sato, Reiko; Morizane, Toshio; Ishii, Hiromasa.
In: Biochemistry and Molecular Biology International, Vol. 42, No. 2, 06.1997, p. 371-379.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Role of H-ras gene in chronic liver damage in mice, by using transgenic mice carrying a human c-H-ras protogene without mutations
AU - Tsunematsu, Satoshi
AU - Saito, Hidetsugu
AU - Sato, Reiko
AU - Morizane, Toshio
AU - Ishii, Hiromasa
PY - 1997/6
Y1 - 1997/6
N2 - Hepatic tumors including hepatocellular carcinoma were generated by carbon tetrachloride in transgenic mice carrying a human c-H-ras gene (rasH2 mice). RasH2 mice express 2 to 3 times more ras protein (ras p21) in the liver than do non-Tg mice. When carbon tetrachloride was administered, the rasH2 mice produced about 5 times as many hepatic tumors than did the non-transgenic mice. However, neither the 10-100 times higher ras p21 expression required for murine fibroblast transformation by itself nor the mutational activation of the H-ras gene was observed in carbon tetrachloride-induced hepatic tumors. These results show that H-ras proto-oncogene expression in the murine liver, even if it is not high enough to transform cells, also causes liver tumors when CCl4 are repeatedly given.
AB - Hepatic tumors including hepatocellular carcinoma were generated by carbon tetrachloride in transgenic mice carrying a human c-H-ras gene (rasH2 mice). RasH2 mice express 2 to 3 times more ras protein (ras p21) in the liver than do non-Tg mice. When carbon tetrachloride was administered, the rasH2 mice produced about 5 times as many hepatic tumors than did the non-transgenic mice. However, neither the 10-100 times higher ras p21 expression required for murine fibroblast transformation by itself nor the mutational activation of the H-ras gene was observed in carbon tetrachloride-induced hepatic tumors. These results show that H-ras proto-oncogene expression in the murine liver, even if it is not high enough to transform cells, also causes liver tumors when CCl4 are repeatedly given.
KW - H-ras
KW - Hepatocellular caricmona
KW - Neoplastic nodule
KW - Over expression
KW - Point mutation
UR - http://www.scopus.com/inward/record.url?scp=0031401930&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031401930&partnerID=8YFLogxK
M3 - Article
VL - 42
SP - 371
EP - 379
JO - IUBMB Life
JF - IUBMB Life
SN - 1521-6543
IS - 2
ER -