Role of HIF-1α-activated Epac1 on HSC-mediated neuroplasticity in stroke model

Chen Huan Lin, Hsu Tung Lee, Shin Da Lee, Wei Lee, Chin Wen Chental Cho, Shinn Zong Lin, Hsiao Jung Wang, Hideyuki Okano, Ching Yuan Su, Yung Luen Yu, Chung Y. Hsu, Woei Cherng Shyu

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Exchange protein activated by cAMP-1 (Epac1) plays an important role in cell proliferation, cell survival and neuronal signaling, and activation of Epac1 in endothelial progenitor cells increases their homing to ischemic muscles and promotes neovascularization in a model of hind limb ischemia. Moreover, upregulation of Epac1 occurs during organ development and in diseases such as myocardial hypertrophy, diabetes, and Alzheimer's disease. We report here that hypoxia upregulated Epac1 through HIF-1α induction in the CD34-immunosorted human umbilical cord blood hematopoietic stem cells (hUCB34). Importantly, implantation of hUCB34 subjected to hypoxia-preconditioning (HP-hUCB34) improved stroke outcome, more than did implantation of untreated hUCB34, in rodents subjected to cerebral ischemia, and this required Epac1-to-matrix metalloprotease (MMP) signaling. This improved therapeutic efficacy correlated with better engraftment and differentiation of these cells in the ischemic host brain. In addition, more than did implantation of untreated HP-hUCB34, implantation of HP-hUCB34 improved cerebral blood flow into the ischemic brain via induction of angiogenesis, facilitated proliferation/recruitment of endogenous neural progenitor cells in the ischemic brain, and promoted neurite outgrowth following cerebral ischemia. Consistent with our proposed role of Epac1-to-MMP signaling in hypoxia-preconditioning, the above mentioned effects of implanting HP-hUCB34 could be abolished by pharmacological inhibition and genetic disruption/deletion of Epac1 or MMPs. We have discovered a HIF-1α-to-Epac1-to-MMP signaling pathway that is required for the improved therapeutic efficacy resulting from hypoxia preconditioning of hUCB34 in vitro prior to their implantation into the host brain in vivo.

Original languageEnglish
Pages (from-to)76-91
Number of pages16
JournalNeurobiology of Disease
Volume58
DOIs
Publication statusPublished - 2013 Oct
Externally publishedYes

Fingerprint

Neuronal Plasticity
Stroke
Proteins
Metalloproteases
Brain
Brain Ischemia
Cerebrovascular Circulation
Hematopoietic Stem Cells
Matrix Metalloproteinases
Fetal Blood
Hypertrophy
Cell Differentiation
Rodentia
Cell Survival
Alzheimer Disease
Up-Regulation
Stem Cells
Ischemia
Extremities
Cell Proliferation

Keywords

  • Cerebral ischemia
  • Epac1
  • HUCB
  • Hypoxia preconditioning (HP)
  • Immunoselection of CD34

ASJC Scopus subject areas

  • Neurology

Cite this

Lin, C. H., Lee, H. T., Lee, S. D., Lee, W., Cho, C. W. C., Lin, S. Z., ... Shyu, W. C. (2013). Role of HIF-1α-activated Epac1 on HSC-mediated neuroplasticity in stroke model. Neurobiology of Disease, 58, 76-91. https://doi.org/10.1016/j.nbd.2013.05.006

Role of HIF-1α-activated Epac1 on HSC-mediated neuroplasticity in stroke model. / Lin, Chen Huan; Lee, Hsu Tung; Lee, Shin Da; Lee, Wei; Cho, Chin Wen Chental; Lin, Shinn Zong; Wang, Hsiao Jung; Okano, Hideyuki; Su, Ching Yuan; Yu, Yung Luen; Hsu, Chung Y.; Shyu, Woei Cherng.

In: Neurobiology of Disease, Vol. 58, 10.2013, p. 76-91.

Research output: Contribution to journalArticle

Lin, CH, Lee, HT, Lee, SD, Lee, W, Cho, CWC, Lin, SZ, Wang, HJ, Okano, H, Su, CY, Yu, YL, Hsu, CY & Shyu, WC 2013, 'Role of HIF-1α-activated Epac1 on HSC-mediated neuroplasticity in stroke model', Neurobiology of Disease, vol. 58, pp. 76-91. https://doi.org/10.1016/j.nbd.2013.05.006
Lin, Chen Huan ; Lee, Hsu Tung ; Lee, Shin Da ; Lee, Wei ; Cho, Chin Wen Chental ; Lin, Shinn Zong ; Wang, Hsiao Jung ; Okano, Hideyuki ; Su, Ching Yuan ; Yu, Yung Luen ; Hsu, Chung Y. ; Shyu, Woei Cherng. / Role of HIF-1α-activated Epac1 on HSC-mediated neuroplasticity in stroke model. In: Neurobiology of Disease. 2013 ; Vol. 58. pp. 76-91.
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AU - Wang, Hsiao Jung

AU - Okano, Hideyuki

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