Role of IL-5 in IL-2-induced eosinophilia: In vivo and in vitro expression of IL-5 mRNA by IL-2 1

Yuji Yamaguchi, Toshio Suda, Hiroko Shiozaki, Yasusada Miura, Yasumichi Hitoshi, Akira Tominaga, Kiyoshi Takatsu, Tadashi Kasahara

Research output: Contribution to journalArticle

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Abstract

We recently demonstrated in vivo that IL-5 was an important mediator of eosinophilia in mice with parasite infections. In this study, we examined whether or not IL-5 was actually responsible for the eosinophilia induced by injection of human rIL-2. Mice administered hIL-2 developed eosinophilia during the course of the series of injections. This eosinophilia could be suppressed by a single injection of mAb against murine IL-5. The number of eosinophilic precursors increased more in the spleen cells of the IL-2-treated mice in comparison to the control mice, although in bone marrow precursors showed little change. Similarly, the number of granulocytic precursors increased markedly in the spleen cells of IL-2-treated mice. In vitro polymerase chain reaction amplification of cDNA subfragments corresponding to IL-5 mRNA (reverse transcription-polymerase chain reaction), followed by Southern blot analysis, revealed enhancement of IL-5 mRNA expression in spleen cells 3 days after starting the human IL-2 injections. We also observed enhanced murine IL-5 mRNA expression in spleen cells stimulated with IL-2 in vitro. The level of murine IL-5 mRNA expression by IL-2-stimulated spleen cells from normal mice increased at 24 h, reached a plateau after 48 h, and decreased again within 72 h of starting culture. These results indicate that the eosinophilia induced by IL-2 in vivo is presumably mediated by IL-5 released from IL-2-stimulated lymphocytes.

Original languageEnglish
Pages (from-to)873-877
Number of pages5
JournalJournal of Immunology
Volume145
Issue number3
Publication statusPublished - 1990 Aug 1

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Interleukin-5
Eosinophilia
Interleukin-1
Interleukin-2
Messenger RNA
Spleen
Injections
Polymerase Chain Reaction
Parasitic Diseases
In Vitro Techniques
Southern Blotting
Reverse Transcription
Complementary DNA
Bone Marrow
Lymphocytes

ASJC Scopus subject areas

  • Immunology

Cite this

Yamaguchi, Y., Suda, T., Shiozaki, H., Miura, Y., Hitoshi, Y., Tominaga, A., ... Kasahara, T. (1990). Role of IL-5 in IL-2-induced eosinophilia: In vivo and in vitro expression of IL-5 mRNA by IL-2 1. Journal of Immunology, 145(3), 873-877.

Role of IL-5 in IL-2-induced eosinophilia : In vivo and in vitro expression of IL-5 mRNA by IL-2 1. / Yamaguchi, Yuji; Suda, Toshio; Shiozaki, Hiroko; Miura, Yasusada; Hitoshi, Yasumichi; Tominaga, Akira; Takatsu, Kiyoshi; Kasahara, Tadashi.

In: Journal of Immunology, Vol. 145, No. 3, 01.08.1990, p. 873-877.

Research output: Contribution to journalArticle

Yamaguchi, Y, Suda, T, Shiozaki, H, Miura, Y, Hitoshi, Y, Tominaga, A, Takatsu, K & Kasahara, T 1990, 'Role of IL-5 in IL-2-induced eosinophilia: In vivo and in vitro expression of IL-5 mRNA by IL-2 1', Journal of Immunology, vol. 145, no. 3, pp. 873-877.
Yamaguchi Y, Suda T, Shiozaki H, Miura Y, Hitoshi Y, Tominaga A et al. Role of IL-5 in IL-2-induced eosinophilia: In vivo and in vitro expression of IL-5 mRNA by IL-2 1. Journal of Immunology. 1990 Aug 1;145(3):873-877.
Yamaguchi, Yuji ; Suda, Toshio ; Shiozaki, Hiroko ; Miura, Yasusada ; Hitoshi, Yasumichi ; Tominaga, Akira ; Takatsu, Kiyoshi ; Kasahara, Tadashi. / Role of IL-5 in IL-2-induced eosinophilia : In vivo and in vitro expression of IL-5 mRNA by IL-2 1. In: Journal of Immunology. 1990 ; Vol. 145, No. 3. pp. 873-877.
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