Role of interferons (IFNs) in the differentiation of T peripheral helper (Tph) cells

Shuhei Tanemura, Hideto Tsujimoto, Noriyasu Seki, Shinji Kojima, Fumihiko Miyoshi, Kunio Sugahara, Keiko Yoshimoto, Katsuya Suzuki, Yuko Kaneko, Kenji Chiba, Tsutomu Takeuchi

Research output: Contribution to journalArticlepeer-review

Abstract

T follicular helper (Tfh) cells and T peripheral helper (Tph) cells produce interleukin (IL)-21 and are thought to contribute to follicular and extra-follicular B-cell activation, respectively, in autoimmune diseases. It is known that programmed cell death-1 (PD-1)-positive CXCR5+ Tfh-like cells are differentiated from human naive CD4+ T cells by IL-12 plus transforming growth factor (TGF)-β. However, it remains unclear what cytokines are required for Tph differentiation. In this study, we found that interferon (IFN)-α and IFN-β reduce the frequency of Tfh-like cells under the IL-12 plus TGF-β condition, whereas they promote generation of PD-1+CXCR5-CD4+ T cells and secretion of IL-21, IFN-γ and CXCL13. Intracellular cytokine staining and T-cell-B-cell co-culture studies indicated that IFN-α promotes generation of IL-21+IFN-γ +CXCR5-CD4+ T cells thereby enhancing B-cell helper function. By IFN-α treatment, the mRNA levels of IL21, IFNG, CXCL13, CD244, SLAMF7, GZMB and PRDM1 were significantly up-regulated but BCL6 mRNA expression was down-regulated, suggesting a Tph-related gene expression pattern. On the other hand, IL-2-neutralization increased mRNA levels of IL21, CXCL13 and CXCR5, retained BCL6, but showed no clear effect on IFNG or PRDM1. RNA sequencing analyses revealed that PD-1hiCXCR5-CD4+ T cells prepared from in vitro culture show a Tph-related gene expression pattern similar with that of PD-1hiCXCR5- Tph cells obtained from the blood of patients with systemic lupus erythematosus. From our findings, it is highly probable that type I IFNs play a key role in differentiation of Tph cells and trigger Tph cell expansion in autoimmune diseases.

Original languageEnglish
Pages (from-to)519-532
Number of pages14
JournalInternational immunology
Volume34
Issue number10
DOIs
Publication statusPublished - 2022 Sep 9

Keywords

  • Autoimmunity
  • CXCL13
  • Human T cell differentiation
  • IFN-γ
  • IL-21

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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