Role of N-cadherin in the regulation of hematopoietic stem cells in the bone marrow niche

Fumio Arai, Kentaro Hosokawa, Hirofumi Toyama, Yoshiko Matsumoto, Toshio Suda

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Cell-cell and cell-extracellular matrix interactions between hematopoietic stem cells (HSCs) and their niches are critical for the maintenance of stem cell properties. Here, it is demonstrated that a cell adhesion molecule, N-cadherin, is expressed in hematopoietic stem/progenitor cells (HSPCs) and plays a critical role in the regulation of HSPC engraftment. Furthermore, overexpression of N-cadherin in HSCs promoted quiescence and preserved HSC activity during serial bone marrow (BM) transplantation (BMT). Inhibition of N-cadherin by the transduction of N-cadherin short hairpin (sh) RNA (shN-cad) reduced the lodgment of donor HSCs to the endosteal surface, resulting in a significant reduction in long-term engraftment. shN-cad-transduced cells were maintained in the spleen for six months after BMT, indicating that N-cadherin expression in HSCs is specifically required in the BM. These findings suggest that N-cadherin-mediated cell adhesion is functionally essential for the regulation of HSPC activities in the BM niche.

Original languageEnglish
Pages (from-to)72-77
Number of pages6
JournalAnnals of the New York Academy of Sciences
Volume1266
Issue number1
DOIs
Publication statusPublished - 2012 Aug

Fingerprint

Cadherins
Hematopoietic Stem Cells
Stem cells
Bone
Bone Marrow
Stem Cells
Niche
Cell adhesion
Stem Cell Niche
Cell Adhesion Molecules
Small Interfering RNA
Cells
Bone Marrow Transplantation
Cell Adhesion
Extracellular Matrix
Spleen
Transplantation
Maintenance

Keywords

  • Hematopoietic stem cells
  • N-cadherin
  • Niche
  • Osteoblasts

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

Cite this

Role of N-cadherin in the regulation of hematopoietic stem cells in the bone marrow niche. / Arai, Fumio; Hosokawa, Kentaro; Toyama, Hirofumi; Matsumoto, Yoshiko; Suda, Toshio.

In: Annals of the New York Academy of Sciences, Vol. 1266, No. 1, 08.2012, p. 72-77.

Research output: Contribution to journalArticle

Arai, Fumio ; Hosokawa, Kentaro ; Toyama, Hirofumi ; Matsumoto, Yoshiko ; Suda, Toshio. / Role of N-cadherin in the regulation of hematopoietic stem cells in the bone marrow niche. In: Annals of the New York Academy of Sciences. 2012 ; Vol. 1266, No. 1. pp. 72-77.
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