Role of oxygen-derived free radicals in fetal growth retardation induced by ischemia-reperfusion in rats

Hitoshi Ishimoto, Michiya Natori, Mamoru Tanaka, Toyohiko Miyazaki, Toshifumi Kobayashi, Yasunori Yoshimura

Research output: Contribution to journalArticle

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Abstract

We investigated the involvement of oxygen-derived free radicals in the pathogenesis of the intrauterine growth retardation (IUGR) induced in Sprague-Dawley rats by ischemia-reperfusion. On day 17 of gestation, rats received saline, superoxide dismutase (SOD, 50,000 U/kg), catalase (CAT, 50,000 U/kg), or SOD + CAT subcutaneously 1 h before induction of 30 min of ischemia of the right uterine horn. On day 21 the placental level of lipid peroxides was significantly increased (P < 0.001 vs. sham-operated group) and IUGR was induced (P < 0.001 vs. left horn) in the saline-treated group (n = 6). Pretreatment with SOD + CAT (n = 6) significantly inhibited the increase in placental lipid peroxides and prevented IUGR. The effect of ischemia- reperfusion on uterine blood flow, with or without pretreatment with radical scavengers, was investigated in separate experiments by laser-Doppler flowmetry. The induction of hypoperfusion 3 h after ischemia (blood flow 40 ± 5%, n = 6, P < 0.05) was blocked by pretreatment with SOD + CAT (n = 6). Results indicate that oxygen-derived free radicals may be important in the development of postischemic uteroplacental hypoperfusion and of ischemia- reperfusion-induced IUGR in the rat.

Original languageEnglish
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume272
Issue number2 41-2
Publication statusPublished - 1997 Feb
Externally publishedYes

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Fetal Growth Retardation
Reperfusion
Free Radicals
Ischemia
Oxygen
Lipid Peroxides
Laser-Doppler Flowmetry
Horns
Catalase
Superoxide Dismutase
Sprague Dawley Rats
Pregnancy

Keywords

  • intrauterine growth retardation
  • lipid peroxides
  • superoxide dismutase catalase
  • uterine blood flow

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Role of oxygen-derived free radicals in fetal growth retardation induced by ischemia-reperfusion in rats. / Ishimoto, Hitoshi; Natori, Michiya; Tanaka, Mamoru; Miyazaki, Toyohiko; Kobayashi, Toshifumi; Yoshimura, Yasunori.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 272, No. 2 41-2, 02.1997.

Research output: Contribution to journalArticle

Ishimoto, Hitoshi ; Natori, Michiya ; Tanaka, Mamoru ; Miyazaki, Toyohiko ; Kobayashi, Toshifumi ; Yoshimura, Yasunori. / Role of oxygen-derived free radicals in fetal growth retardation induced by ischemia-reperfusion in rats. In: American Journal of Physiology - Heart and Circulatory Physiology. 1997 ; Vol. 272, No. 2 41-2.
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