TY - JOUR
T1 - Role of P-glycoprotein in all-trans retinoic acid (ATRA) resistance in acute promyelocytic leukaemia cells
T2 - Analysis of intracellular concentration of ATRA
AU - Takeshita, Akihiro
AU - Shinjo, Kaori
AU - Naito, Kensuke
AU - Ohnishi, Kazunori
AU - Sugimoto, Yoshikazu
AU - Yamakawa, Yoshinori
AU - Tanimoto, Mitsune
AU - Kitamura, Kunio
AU - Naoe, Tumoki
AU - Ohno, Ryuzo
PY - 2000
Y1 - 2000
N2 - We analysed the relationship between all-trans retinoic acid (ATRA) resistance and P-glycoprotein (P-gp)-associated multidrug resistance (MDR) in acute promyelocytic leukaemia (APL). There was no difference in the intracellular ATRA accumulation between NB4 cells and an MDR1 cDNA-transduced NB4 subline and between ATRA-resistant NB4 cells (NB4/RA) and an MDR1 cDNA- transduced NB4/RA subline. PSC833, a MDR modifier, did not increase the intracellular accumulation of ATRA or affect the expression of CD11b, the nitroblue tetrazolium (NBT) reduction activity, the proportion of apoptotic cells or the morphology of these four ATRA-treated cell lines. Similar results were obtained in the analysis of APL cells from five patients relapsed after ATRA-induced complete remission.
AB - We analysed the relationship between all-trans retinoic acid (ATRA) resistance and P-glycoprotein (P-gp)-associated multidrug resistance (MDR) in acute promyelocytic leukaemia (APL). There was no difference in the intracellular ATRA accumulation between NB4 cells and an MDR1 cDNA-transduced NB4 subline and between ATRA-resistant NB4 cells (NB4/RA) and an MDR1 cDNA- transduced NB4/RA subline. PSC833, a MDR modifier, did not increase the intracellular accumulation of ATRA or affect the expression of CD11b, the nitroblue tetrazolium (NBT) reduction activity, the proportion of apoptotic cells or the morphology of these four ATRA-treated cell lines. Similar results were obtained in the analysis of APL cells from five patients relapsed after ATRA-induced complete remission.
KW - Acute promyelocytic leukaemia
KW - All-trans retinoic acid
KW - Multidrug resistance
KW - P-glycoprotein
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U2 - 10.1046/j.1365-2141.2000.01823.x
DO - 10.1046/j.1365-2141.2000.01823.x
M3 - Article
C2 - 10651729
AN - SCOPUS:0033954405
SN - 0007-1048
VL - 108
SP - 90
EP - 92
JO - British Journal of Haematology
JF - British Journal of Haematology
IS - 1
ER -