Role of phosphoenolpyruvate in the NADP-isocitrate dehydrogenase and isocitrate lyase reaction in Escherichia coli

Tadashi Ogawa, Keiko Murakami, Hirotada Mori, Nobuyoshi Ishii, Masaru Tomita, Masataka Yoshin

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Phosphoenolpyruvate inhibited Escherichia coli NADP-isocitrate dehydrogenase allosterically (Ki of 0.31 mM) and isocitrate lyase uncompetitively (Ki′ of 0.893 mM). Phosphoenolpyruvate enhances the uncompetitive inhibition of isocitrate lyase by increasing isocitrate, which protects isocitrate dehydrogenase from the inhibition, and contributes to the control through the tricarboxylic acid cycle and glyoxylate shunt.

Original languageEnglish
Pages (from-to)1176-1178
Number of pages3
JournalJournal of Bacteriology
Issue number3
Publication statusPublished - 2007 Feb


ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

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