Role of phosphoenolpyruvate in the NADP-isocitrate dehydrogenase and isocitrate lyase reaction in Escherichia coli

Tadashi Ogawa, Keiko Murakami, Hirotada Mori, Nobuyoshi Ishii, Masaru Tomita, Masataka Yoshin

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Phosphoenolpyruvate inhibited Escherichia coli NADP-isocitrate dehydrogenase allosterically (Ki of 0.31 mM) and isocitrate lyase uncompetitively (Ki′ of 0.893 mM). Phosphoenolpyruvate enhances the uncompetitive inhibition of isocitrate lyase by increasing isocitrate, which protects isocitrate dehydrogenase from the inhibition, and contributes to the control through the tricarboxylic acid cycle and glyoxylate shunt.

Original languageEnglish
Pages (from-to)1176-1178
Number of pages3
JournalJournal of Bacteriology
Volume189
Issue number3
DOIs
Publication statusPublished - 2007 Feb 1

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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