Role of protein kinase A in regulating steroid sulfate uptake for estrogen production in human placental choriocarcinoma cells

M. Tomi, Y. Miyata, S. Noguchi, S. Nishimura, T. Nishimura, E. Nakashima

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The purpose of this study is to assess the role of the protein kinase A (PKA) in regulating uptake of dehydroepiandrosterone sulfate (DHEAS), an estrogen precursor, by syncytiotrophoblasts. Forskolin, a PKA activator, significantly increased [3H]DHEAS uptake and the mRNA expression levels of organic anion transporter (OAT) 4 and CYP19A1 in choriocarcinoma JEG-3 cells, while other steroid sulfate transporters present in the placenta showed no change in expression level. KT5720, a PKA inhibitor, attenuated these effects of forskolin. Accordingly, the PKA pathway appears to play an important role in estrogen synthesis by cooperatively regulating OAT4 and steroidogenic enzymes in syncytiotrophoblasts.

Original languageEnglish
Pages (from-to)658-660
Number of pages3
JournalPlacenta
Volume35
Issue number8
DOIs
Publication statusPublished - 2014 Aug

Keywords

  • Dehydroepiandrosterone sulfate
  • Estrogen synthesis
  • OAT4
  • Protein kinase A
  • Syncytiotrophoblast

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynaecology
  • Developmental Biology

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