Role of Steroid Administration to Reduce Inflammation After Thoracotomy in a Rat Surgical Stress Model

Eiichi Nakamura, Yuukou Kitagawa, Soji Ozawa, Koichi Suda, Nobutoshi Ando, Masakazu Ueda, Masaki Kitajima

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background: Unilateral lung surgical insult can lead to bilateral pulmonary inflammation after transthoracic esophagectomy for esophageal cancer. An inflammatory response of the cytokine network induced by surgical stress followed by neutrophil sequestration is the major mechanism involved in these complications. We examined modulation of the inflammatory cytokine and neutrophil sequestration by preoperative steroid administration in a rat thoracotomy model. Methods: Adult male rats were subjected to right thoracotomy with pulmonary compression in the surgical stress model. In the another surgical stress model, 10 mg/kg of methylprednisolone (MP) was administered 1 h before the operation intraperitoneally, and peri-operative biological effects of steroid administration to neutrophil sequestration and cytokine-induced neutrophil chemoattractant (CINC) as an inflammatory cytokine were examined. Sham-operated animals were subjected to intubation only. Results: Surgical insult to the unilateral lung increased local CINC concentration followed by an increase in neutrophil sequestration, wet-to-dry weight ratio in bilateral lungs and systemic CINC concentration. Pre-treatment with methylprednisolone reduced CINC concentration as well as neutrophil sequestration and wet-to-dry weight ratio of both lungs. Conclusions: These data suggest that inflammatory changes induced by the unilateral operative procedure occur in both lungs through local inflammatory response. Pre-operative methylprednisolone administration down-regulates cytokine release and neutrophil sequestration, preventing lung edema, and possibly may act as a prophylactic treatment against acute lung injury caused by a so-called "second attack" theory.

Original languageEnglish
Pages (from-to)364-369
Number of pages6
JournalJournal of Surgical Research
Volume135
Issue number2
DOIs
Publication statusPublished - 2006 Oct

Fingerprint

Anatomic Models
Thoracotomy
Neutrophils
Steroids
Inflammation
Cytokines
Chemotactic Factors
Lung
Methylprednisolone
Weights and Measures
Esophagectomy
Acute Lung Injury
Operative Surgical Procedures
Esophageal Neoplasms
Intubation
Edema
Pneumonia
Down-Regulation

Keywords

  • inflammation
  • rat
  • reduce
  • steroid
  • surgical stress
  • thoracotomy

ASJC Scopus subject areas

  • Surgery

Cite this

Role of Steroid Administration to Reduce Inflammation After Thoracotomy in a Rat Surgical Stress Model. / Nakamura, Eiichi; Kitagawa, Yuukou; Ozawa, Soji; Suda, Koichi; Ando, Nobutoshi; Ueda, Masakazu; Kitajima, Masaki.

In: Journal of Surgical Research, Vol. 135, No. 2, 10.2006, p. 364-369.

Research output: Contribution to journalArticle

Nakamura, Eiichi ; Kitagawa, Yuukou ; Ozawa, Soji ; Suda, Koichi ; Ando, Nobutoshi ; Ueda, Masakazu ; Kitajima, Masaki. / Role of Steroid Administration to Reduce Inflammation After Thoracotomy in a Rat Surgical Stress Model. In: Journal of Surgical Research. 2006 ; Vol. 135, No. 2. pp. 364-369.
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AB - Background: Unilateral lung surgical insult can lead to bilateral pulmonary inflammation after transthoracic esophagectomy for esophageal cancer. An inflammatory response of the cytokine network induced by surgical stress followed by neutrophil sequestration is the major mechanism involved in these complications. We examined modulation of the inflammatory cytokine and neutrophil sequestration by preoperative steroid administration in a rat thoracotomy model. Methods: Adult male rats were subjected to right thoracotomy with pulmonary compression in the surgical stress model. In the another surgical stress model, 10 mg/kg of methylprednisolone (MP) was administered 1 h before the operation intraperitoneally, and peri-operative biological effects of steroid administration to neutrophil sequestration and cytokine-induced neutrophil chemoattractant (CINC) as an inflammatory cytokine were examined. Sham-operated animals were subjected to intubation only. Results: Surgical insult to the unilateral lung increased local CINC concentration followed by an increase in neutrophil sequestration, wet-to-dry weight ratio in bilateral lungs and systemic CINC concentration. Pre-treatment with methylprednisolone reduced CINC concentration as well as neutrophil sequestration and wet-to-dry weight ratio of both lungs. Conclusions: These data suggest that inflammatory changes induced by the unilateral operative procedure occur in both lungs through local inflammatory response. Pre-operative methylprednisolone administration down-regulates cytokine release and neutrophil sequestration, preventing lung edema, and possibly may act as a prophylactic treatment against acute lung injury caused by a so-called "second attack" theory.

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