Role of the amino-terminal region of the DnaA protein in opening of the duplex DNA at the oriC region

Shinji Mima; Yoshihiro Yamagachi; Taemi Kondo; Tomofusa Tsuchiya; Tohru Mizushima

doi:10.1016/S0378-1097(99)00233-5

Role of the amino-terminal region of the DnaA protein in opening of the duplex DNA at the oriC region

Shinji Mima, Yoshihiro Yamagachi, Taemi Kondo, Tomofusa Tsuchiya, Tohru Mizushima

School of Pharmacy

Research output: Contribution to journal › Article

9 Citations (Scopus)

Abstract

We report in this paper that the amino acid residues Ile-26 and Leu-40 of the DnaA protein are essential for the DNA replication activity in vitro. Lines of evidence to support this conclusion are as follows. Variants of the DnaA protein containing either an Ile-26-Ser or Leu-40-Ser replacement were unable to support oriC DNA replication in vitro. Though the mutant DnaA proteins retained the capability to bind oriC DNA, they were unable to open the duplex DNA at oriC. Based on these and other results, we conclude that the N-terminal region of the DnaA, protein is involved in the oligomerization of this protein, an essential step for the duplex opening activity at oriC.

Original language	English
Pages (from-to)	163-167
Number of pages	5
Journal	FEMS microbiology letters
Volume	176
Issue number	1
DOIs	https://doi.org/10.1016/S0378-1097(99)00233-5
Publication status	Published - 1999 Jul 1

Keywords

DNA replication
DnaA
N-terminal region
Site-directed mutagenesis

ASJC Scopus subject areas

Microbiology
Molecular Biology
Genetics

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Mima, S., Yamagachi, Y., Kondo, T., Tsuchiya, T., & Mizushima, T. (1999). Role of the amino-terminal region of the DnaA protein in opening of the duplex DNA at the oriC region. FEMS microbiology letters, 176(1), 163-167. https://doi.org/10.1016/S0378-1097(99)00233-5

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abstract = "We report in this paper that the amino acid residues Ile-26 and Leu-40 of the DnaA protein are essential for the DNA replication activity in vitro. Lines of evidence to support this conclusion are as follows. Variants of the DnaA protein containing either an Ile-26-Ser or Leu-40-Ser replacement were unable to support oriC DNA replication in vitro. Though the mutant DnaA proteins retained the capability to bind oriC DNA, they were unable to open the duplex DNA at oriC. Based on these and other results, we conclude that the N-terminal region of the DnaA, protein is involved in the oligomerization of this protein, an essential step for the duplex opening activity at oriC.",

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AU - Mima, Shinji

AU - Yamagachi, Yoshihiro

AU - Kondo, Taemi

AU - Tsuchiya, Tomofusa

AU - Mizushima, Tohru

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N2 - We report in this paper that the amino acid residues Ile-26 and Leu-40 of the DnaA protein are essential for the DNA replication activity in vitro. Lines of evidence to support this conclusion are as follows. Variants of the DnaA protein containing either an Ile-26-Ser or Leu-40-Ser replacement were unable to support oriC DNA replication in vitro. Though the mutant DnaA proteins retained the capability to bind oriC DNA, they were unable to open the duplex DNA at oriC. Based on these and other results, we conclude that the N-terminal region of the DnaA, protein is involved in the oligomerization of this protein, an essential step for the duplex opening activity at oriC.

AB - We report in this paper that the amino acid residues Ile-26 and Leu-40 of the DnaA protein are essential for the DNA replication activity in vitro. Lines of evidence to support this conclusion are as follows. Variants of the DnaA protein containing either an Ile-26-Ser or Leu-40-Ser replacement were unable to support oriC DNA replication in vitro. Though the mutant DnaA proteins retained the capability to bind oriC DNA, they were unable to open the duplex DNA at oriC. Based on these and other results, we conclude that the N-terminal region of the DnaA, protein is involved in the oligomerization of this protein, an essential step for the duplex opening activity at oriC.

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KW - N-terminal region

KW - Site-directed mutagenesis

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