Roles for the Yb body components Armitage and Yb in primary piRNA biogenesis in Drosophila

Kuniaki Saito, Hirotsugu Ishizu, Miharu Komai, Hazuki Kotani, Yoshinori Kawamura, Kazumichi M. Nishida, Haruhiko Siomi, Mikiko C. Siomi

Research output: Contribution to journalArticle

197 Citations (Scopus)

Abstract

PIWI-interacting RNAs (piRNAs) protect genome integrity from transposons. In Drosophila ovarian somas, primary piRNAs are produced and loaded onto Piwi. Here, we describe roles for the cytoplasmic Yb body components Armitage and Yb in somatic primary piRNA biogenesis. Armitage binds to Piwi and is required for localizing Piwi into Yb bodies. Without Armitage or Yb, Piwi is freed from the piRNAs and does not enter the nucleus. Thus, piRNA loading is required for Piwi nuclear entry. We propose that a functional Piwi-piRNA complex is formed and inspected in Yb bodies before its nuclear entry to exert transposon silencing.

Original languageEnglish
Pages (from-to)2493-2498
Number of pages6
JournalGenes and Development
Volume24
Issue number22
DOIs
Publication statusPublished - 2010 Nov 15

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Small Interfering RNA
Drosophila
RNA
Carisoprodol
Genome

Keywords

  • Armitage
  • Drosophila
  • Piwi
  • Primary piRNA
  • Yb
  • Zucchini

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

Saito, K., Ishizu, H., Komai, M., Kotani, H., Kawamura, Y., Nishida, K. M., ... Siomi, M. C. (2010). Roles for the Yb body components Armitage and Yb in primary piRNA biogenesis in Drosophila. Genes and Development, 24(22), 2493-2498. https://doi.org/10.1101/gad.1989510

Roles for the Yb body components Armitage and Yb in primary piRNA biogenesis in Drosophila. / Saito, Kuniaki; Ishizu, Hirotsugu; Komai, Miharu; Kotani, Hazuki; Kawamura, Yoshinori; Nishida, Kazumichi M.; Siomi, Haruhiko; Siomi, Mikiko C.

In: Genes and Development, Vol. 24, No. 22, 15.11.2010, p. 2493-2498.

Research output: Contribution to journalArticle

Saito, K, Ishizu, H, Komai, M, Kotani, H, Kawamura, Y, Nishida, KM, Siomi, H & Siomi, MC 2010, 'Roles for the Yb body components Armitage and Yb in primary piRNA biogenesis in Drosophila', Genes and Development, vol. 24, no. 22, pp. 2493-2498. https://doi.org/10.1101/gad.1989510
Saito, Kuniaki ; Ishizu, Hirotsugu ; Komai, Miharu ; Kotani, Hazuki ; Kawamura, Yoshinori ; Nishida, Kazumichi M. ; Siomi, Haruhiko ; Siomi, Mikiko C. / Roles for the Yb body components Armitage and Yb in primary piRNA biogenesis in Drosophila. In: Genes and Development. 2010 ; Vol. 24, No. 22. pp. 2493-2498.
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