Roles of Cbln1 in non-motor functions of mice

Shintaro Otsuka, Kohtarou Konno, Manabu Abe, Junko Motohashi, Kazuhisa Kohda, Kenji Sakimura, Masahiko Watanabe, Michisuke Yuzaki

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The cerebellum is thought to be involved in cognitive functions in addition to its well established role in motor coordination and motor learning in humans. Cerebellin 1 (Cbln1) is predominantly expressed in cerebellar granule cells and plays a crucial role in the formation and function of parallel fiber–Purkinje cell synapses. Although genes encoding Cbln1 and its postsynaptic receptor,the delta2 glutamate receptor (GluD2),are suggested to be associated with autistic-like traits and many psychiatric disorders,whether such cognitive impairments are caused by cerebellar dysfunction remains unclear. In the present study,we investigated whether and how Cbln1 signaling is involved in non-motor functions in adult mice. We show that acquisition and retention/retrieval of cued and contextual fear memory were impaired in Cbln1-null mice. In situ hybridization and immunohistochemical analyses revealed that Cbln1 is expressed in various extracerebellar regions,including the retrosplenial granular cortex and the hippocampus. In the hippocampus,Cbln1 immunoreactivity was present at the molecular layer of the dentate gyrus and the stratum lacunosum-moleculare without overt mRNA expression,suggesting that Cbln1 is provided by perforant path fibers. Retention/retrieval,but not acquisition,of cued and contextual fear memory was impaired in forebrain-predominant Cbln1-null mice. Spatial learning in the radial arm water maze was also abrogated. In contrast,acquisition of fear memory was affected in cerebellum-predominant Cbln1-null mice. These results indicate that Cbln1 in the forebrain and cerebellum mediates specific aspects of fear conditioning and spatial memory differentially and that Cbln1 signaling likely regulates motor and non-motor functions in multiple brain regions.

Original languageEnglish
Pages (from-to)11801-11816
Number of pages16
JournalJournal of Neuroscience
Volume36
Issue number46
DOIs
Publication statusPublished - 2016 Nov 16

Fingerprint

Fear
Cerebellum
Prosencephalon
Hippocampus
cerebellin
Perforant Pathway
Cerebellar Diseases
Dentate Gyrus
Synapses
Cognition
In Situ Hybridization
Psychiatry
Learning
Messenger RNA
Water
Brain
Genes
Retention (Psychology)

Keywords

  • Cerebellum
  • Fear conditioning
  • Hippocampus
  • Retrosplenial granular cortex
  • Synapse formation

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Otsuka, S., Konno, K., Abe, M., Motohashi, J., Kohda, K., Sakimura, K., ... Yuzaki, M. (2016). Roles of Cbln1 in non-motor functions of mice. Journal of Neuroscience, 36(46), 11801-11816. https://doi.org/10.1523/JNEUROSCI.0322-16.2016

Roles of Cbln1 in non-motor functions of mice. / Otsuka, Shintaro; Konno, Kohtarou; Abe, Manabu; Motohashi, Junko; Kohda, Kazuhisa; Sakimura, Kenji; Watanabe, Masahiko; Yuzaki, Michisuke.

In: Journal of Neuroscience, Vol. 36, No. 46, 16.11.2016, p. 11801-11816.

Research output: Contribution to journalArticle

Otsuka, S, Konno, K, Abe, M, Motohashi, J, Kohda, K, Sakimura, K, Watanabe, M & Yuzaki, M 2016, 'Roles of Cbln1 in non-motor functions of mice', Journal of Neuroscience, vol. 36, no. 46, pp. 11801-11816. https://doi.org/10.1523/JNEUROSCI.0322-16.2016
Otsuka S, Konno K, Abe M, Motohashi J, Kohda K, Sakimura K et al. Roles of Cbln1 in non-motor functions of mice. Journal of Neuroscience. 2016 Nov 16;36(46):11801-11816. https://doi.org/10.1523/JNEUROSCI.0322-16.2016
Otsuka, Shintaro ; Konno, Kohtarou ; Abe, Manabu ; Motohashi, Junko ; Kohda, Kazuhisa ; Sakimura, Kenji ; Watanabe, Masahiko ; Yuzaki, Michisuke. / Roles of Cbln1 in non-motor functions of mice. In: Journal of Neuroscience. 2016 ; Vol. 36, No. 46. pp. 11801-11816.
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