Roles of ICAM-1 for abnormal leukocyte recruitment in the microcirculation of bleomycin-induced fibrotic lung injury

Nagato Sato, Yukio Suzuki, Kazumi Nishio, Koichi Suzuki, Katsuhiko Naoki, Kei Takeshita, Hiroyasu Kudo, Naoki Miyao, Harukuni Tsumura, Hiroshi Serizawa, Makoto Suematsu, Kazuhiro Yamaguchi

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Abstract

To assess the importance of endothelial intercellular adhesion molecule- 1 (ICAM-1) in microvascular leukocyte kinetics in diseased lungs, we investigated the transitional changes in ICAM-1 expression, vascular diameter, and leukocyte behavior in rat pulmonary microcirculation during the development of acute lung injury (ALI) and chronic fibrosis (FIB) evoked by bleomycin (BLM). Observations were made in the isolated perfused lung with a real-time confocal laser luminescence microscope. Microvascular cell kinetics were evaluated by measuring the behavior of fluorescence-labeled leukocytes and erythrocytes in the presence or absence of anti-ICAM-1 monoclonal antibody (1A29). Arteriolar ICAM-1 showed little change at any time after BLM treatment. Venular ICAM-1 was first enhanced at the initial phase of ALI followed by the second upregulation at the early phase of FIB. Capillary ICAM-1 showed a sustained increase at both ALI and FIB. Arteriolar and venule diameters were not altered but capillary diameter decreased during ALI and early FIB stages. Although firm adherence of leukocytes to arteriolar and venular walls was not observed, rolling leukocytes were increased in venules both at the initial phase of ALI and at the early phase of FIB. The leukocyte rolling in venules correlated well with transitional changes in ICAM-1 and was inhibited by 1A29. Sustained entrapment of leukocytes in capillaries was attributed to changes in vascular diameter as well as augmented ICAM-1. In conclusion, ICAM-1 plays an important role in microvascular leukocyte recruitment in both ALI and FIB in the BLM-injured lung.

Original languageEnglish
Pages (from-to)1681-1688
Number of pages8
JournalAmerican Journal of Respiratory and Critical Care Medicine
Volume161
Issue number5
Publication statusPublished - 2000

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Lung Injury
Intercellular Adhesion Molecule-1
Microcirculation
Leukocytes
Acute Lung Injury
Fibrosis
Venules
Leukocyte Rolling
Bleomycin
Lung
Blood Vessels
indium-bleomycin
Luminescence
Lung Diseases
Lasers
Up-Regulation
Erythrocytes
Fluorescence
Monoclonal Antibodies

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Sato, N., Suzuki, Y., Nishio, K., Suzuki, K., Naoki, K., Takeshita, K., ... Yamaguchi, K. (2000). Roles of ICAM-1 for abnormal leukocyte recruitment in the microcirculation of bleomycin-induced fibrotic lung injury. American Journal of Respiratory and Critical Care Medicine, 161(5), 1681-1688.

Roles of ICAM-1 for abnormal leukocyte recruitment in the microcirculation of bleomycin-induced fibrotic lung injury. / Sato, Nagato; Suzuki, Yukio; Nishio, Kazumi; Suzuki, Koichi; Naoki, Katsuhiko; Takeshita, Kei; Kudo, Hiroyasu; Miyao, Naoki; Tsumura, Harukuni; Serizawa, Hiroshi; Suematsu, Makoto; Yamaguchi, Kazuhiro.

In: American Journal of Respiratory and Critical Care Medicine, Vol. 161, No. 5, 2000, p. 1681-1688.

Research output: Contribution to journalArticle

Sato, N, Suzuki, Y, Nishio, K, Suzuki, K, Naoki, K, Takeshita, K, Kudo, H, Miyao, N, Tsumura, H, Serizawa, H, Suematsu, M & Yamaguchi, K 2000, 'Roles of ICAM-1 for abnormal leukocyte recruitment in the microcirculation of bleomycin-induced fibrotic lung injury', American Journal of Respiratory and Critical Care Medicine, vol. 161, no. 5, pp. 1681-1688.
Sato, Nagato ; Suzuki, Yukio ; Nishio, Kazumi ; Suzuki, Koichi ; Naoki, Katsuhiko ; Takeshita, Kei ; Kudo, Hiroyasu ; Miyao, Naoki ; Tsumura, Harukuni ; Serizawa, Hiroshi ; Suematsu, Makoto ; Yamaguchi, Kazuhiro. / Roles of ICAM-1 for abnormal leukocyte recruitment in the microcirculation of bleomycin-induced fibrotic lung injury. In: American Journal of Respiratory and Critical Care Medicine. 2000 ; Vol. 161, No. 5. pp. 1681-1688.
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AU - Sato, Nagato

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AU - Suzuki, Koichi

AU - Naoki, Katsuhiko

AU - Takeshita, Kei

AU - Kudo, Hiroyasu

AU - Miyao, Naoki

AU - Tsumura, Harukuni

AU - Serizawa, Hiroshi

AU - Suematsu, Makoto

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N2 - To assess the importance of endothelial intercellular adhesion molecule- 1 (ICAM-1) in microvascular leukocyte kinetics in diseased lungs, we investigated the transitional changes in ICAM-1 expression, vascular diameter, and leukocyte behavior in rat pulmonary microcirculation during the development of acute lung injury (ALI) and chronic fibrosis (FIB) evoked by bleomycin (BLM). Observations were made in the isolated perfused lung with a real-time confocal laser luminescence microscope. Microvascular cell kinetics were evaluated by measuring the behavior of fluorescence-labeled leukocytes and erythrocytes in the presence or absence of anti-ICAM-1 monoclonal antibody (1A29). Arteriolar ICAM-1 showed little change at any time after BLM treatment. Venular ICAM-1 was first enhanced at the initial phase of ALI followed by the second upregulation at the early phase of FIB. Capillary ICAM-1 showed a sustained increase at both ALI and FIB. Arteriolar and venule diameters were not altered but capillary diameter decreased during ALI and early FIB stages. Although firm adherence of leukocytes to arteriolar and venular walls was not observed, rolling leukocytes were increased in venules both at the initial phase of ALI and at the early phase of FIB. The leukocyte rolling in venules correlated well with transitional changes in ICAM-1 and was inhibited by 1A29. Sustained entrapment of leukocytes in capillaries was attributed to changes in vascular diameter as well as augmented ICAM-1. In conclusion, ICAM-1 plays an important role in microvascular leukocyte recruitment in both ALI and FIB in the BLM-injured lung.

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